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Comparison of gene expression between Human umbilical vein endothelial cells and Human liver sinusoidal endothelial cells

ABSTRACT: To identify leukocyte adhesion receptors which differentially regulate recruitment in human liver sinusoidal endothelial cells compared to a protoptypic venular endothelium Gene expression was measured in four groups Group 1: cultured human liver sinusoidal endothelial cells (HSEC) Group 2: cultured human umbilical vein endothelial cells (HUVEC) Group3: Interferon gamma and tumour necrosisfactor alpha treated HSEC and Group 4: Interferon gamma and tumour necrosisfactor alpha treated HUVEC. Two replicates were used for each group.

ORGANISM(S): Homo sapiens

SUBMITTER: Shishir Shetty

PROVIDER: E-GEOD-78020 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Human liver sinusoidal endothelial cells promote intracellular crawling of lymphocytes during recruitment: A new step in migration.

Patten Daniel A DA Wilson Garrick K GK Bailey Dalan D Shaw Robert K RK Jalkanen Sirpa S Salmi Marko M Rot Antal A Weston Chris J CJ Adams David H DH Shetty Shishir S

Hepatology (Baltimore, Md.) 20161125 1

The recruitment of lymphocytes via the hepatic sinusoidal channels and positioning within liver tissue is a critical event in the development and persistence of chronic inflammatory liver diseases. The hepatic sinusoid is a unique vascular bed lined by hepatic sinusoidal endothelial cells (HSECs), a functionally and phenotypically distinct subpopulation of endothelial cells. Using flow-based adhesion assays to study the migration of lymphocytes across primary human HSECs, we found that lymphocyt ...[more]

PMID: 27770554

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