Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Cell wide response to low oxygen exposure in Desulfovibrio vulgaris Hildenborough

ABSTRACT: We investigated the effects of low level oxygen on Desulfovibrio vulgaris Hildenborough using microarrays. Keywords: time course, stress response Comparison of cells treated with 1000 ppm oxygen to untreated cells at 60,120,240 min.

ORGANISM(S): Desulfovibrio vulgaris str. Hildenborough

SUBMITTER: Marcin Joachimiak

PROVIDER: E-GEOD-7816 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Cell-wide responses to low-oxygen exposure in Desulfovibrio vulgaris Hildenborough.

Mukhopadhyay Aindrila A Redding Alyssa M AM Joachimiak Marcin P MP Arkin Adam P AP Borglin Sharon E SE Dehal Paramvir S PS Chakraborty Romy R Geller Jil T JT Hazen Terry C TC He Qiang Q Joyner Dominique C DC Martin Vincent J J VJ Wall Judy D JD Yang Zamin Koo ZK Zhou Jizhong J Keasling Jay D JD

Journal of bacteriology 20070601 16

The responses of the anaerobic, sulfate-reducing organism Desulfovibrio vulgaris Hildenborough to low-oxygen exposure (0.1% O(2)) were monitored via transcriptomics and proteomics. Exposure to 0.1% O(2) caused a decrease in the growth rate without affecting viability. Concerted upregulation of the predicted peroxide stress response regulon (PerR) genes was observed in response to the 0.1% O(2) exposure. Several of the candidates also showed increases in protein abundance. Among the remaining sma ...[more]

PMID: 17545284

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Project description:Background. Desulfovibrio vulgaris Hildenborough is a sulfate-reducing bacterium (SRB) that is intensively studied in the context of metal corrosion and heavy-metal bioremediation, and SRB populations are commonly observed in pipe and subsurface environments as surface-associated populations. In order to elucidate physiological changes associated with biofilm growth at both the transcript and protein level, transcriptomic and proteomic analyses were done on mature biofilm cells and compared to both batch and reactor planktonic populations. The biofilms were cultivated with lactate and sulfate in a continiouslly fed biofilm reactor, and compared to both batch and reactor planktonic populations. The functional genomic analysis demonstrated that biofilm cells were different compared to planktonic cells, and the majority of altered abundances for genes and proteins were annotated as hypothetical (unknown function), energy conservation, amino acid metabolism, and signal transduction. Genes and proteins that showed similar trends in detected levels were particularly involved in energy conservation such as increases in an annotated ech hydrogenase, formate dehydrogenase, pyruvate:ferredoxin oxidoreductase, and rnf oxidoreductase, and the biofilm cells had elevated formate dehydrogenase activity. Several other hydrogenases and formate dehydrogenases also showed an increased protein level, while decreased transcript and protein levels were observed for putative coo hydrogenases as well as a lactate permease and hyp hydrogenases for biofilm cells. Genes annotated for amino acid synthesis and nitrogen utilization were also predominant changers within the biofilm state. Ribosomal transcripts and proteins were notably decreased within the biofilm cells compared to exponential-phase cells but were not as low as levels observed in planktonic, stationary-phase cells. Several putative, extracellular proteins (DVU1012, 1545) were also detected in the extracellular fraction from biofilm cells. Even though both the planktonic and biofilm cells were oxidizing lactate and reducing sulfate, the biofilm cells were physiologically distinct compared to planktonic growth states due to altered abundances of genes/proteins involved in carbon/energy flow and extracellular structures. In addition, average expression values for multiple rRNA transcripts and respiratory activity measurements indicated that biofilm cells were metabolically more similar to exponential-phase cells although biofilm cells are structured differently. The characterization of physiological advantages and constraints of the biofilm growth state for sulfate-reducing bacteria will provide insight into bioremediation applications as well as microbially-induced metal corrosion. Biofilms grown in reactors were compared to reference samples of reactor, planktonic and batch, planktonic. Each sample had a biological triplicate.

Dataset Information

Cell wide response to low oxygen exposure in Desulfovibrio vulgaris Hildenborough

Publications

Cell-wide responses to low-oxygen exposure in Desulfovibrio vulgaris Hildenborough.

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