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Unknown,Transcriptomics,Genomics,Proteomics

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Gene expression analysis in NB adherent primary cell lines after endothelin-1 treatment

ABSTRACT: We cultured adherent primary cell lines from NB tumor samples. Adherent primary cell lines from NB tumor samples have a subpopulation of neural crest progenitors that grow as spheres when cultured in low-binding conditions. We tested the changes in gene expression induced by endothelin-1 (ET1), a cytokine that regulates proliferation, migration, differentiation and survival of NC cells .

ORGANISM(S): Homo sapiens

SUBMITTER: Ricardo Pardal

PROVIDER: E-GEOD-78268 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Gene expression analysis in NB adherent primary cell lines after endothelin-1 treatment

Project description:We cultured adherent primary cell lines from NB tumor samples. Adherent primary cell lines from NB tumor samples have a subpopulation of neural crest progenitors that grow as spheres when cultured in low-binding conditions.

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Expression data of 3 prostate cancer stem cell primary lines comparing spheres and parental/adherent culture

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2021-11-02 | PXD027187 | Pride

Effect of endothelin-1 (ET-1) on mouse testicular peritubular myoid cells [miRNA-Seq]

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Effect of endothelin-1 (ET-1) on mouse testicular peritubular myoid cells [RNA-Seq]

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Transcription profiling of mouse dermal fibroblasts following exposure to endothelin-1

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2012-06-19 | E-GEOD-31145 | biostudies-arrayexpress

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Project description:Mouse primary dermal fibroblasts were treated with 100 nM endothelin-1 (ET1) synthetic peptide for 24 hours. Control samples received no ET1 peptide. The experiment compared treated to untreated to identify gene expression changes due to ET1 exposure. There are three biological replicates for both control and treated samples. These biological replicates represent separate derivations of primary dermal fibroblasts from genetically identical mouse litters aged 0-3 days.

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Gene expression analisys of GD2+ cells from metastatic NB, unselected bone marrow from metastatic NB, primary tumors from stage 4 NB (alive and dead at 5 years of follow-up) and primary tumor from localized NB

Project description:Metastases in the bone marrow (BM) are grim prognostic factors in patients with neuroblastoma (NB). In spite of extensive analysis of primary tumor cells from high- and low-risk NB patients, a characterization of freshly isolated BM-infiltrating metastatic NB cells is still lacking. Our aim was to identify proteins specifically expressed by metastatic NB cells, that may be relevant for prognostic and therapeutic purposes. Metastatic NB cells were freshly isolated from patients’ BM by positive immunomagnetic bead manipulation using anti-GD2 monoclonal antibody. Unselected BM samples from patients with metastatic NB were also included. Gene expression profiles were compared with those obtained from archived NB primary tumors from patients with 5y-follow-up. After validation by RT-qPCR, expression/secretion of the proteins encoded by the up-regulated genes in the BM-infiltrating NB cells was evaluated by flow cytometry and ELISA. Compared to primary tumor cells, BM-infiltrating NB cells down-modulated the expression of CX3CL1, AGT, ATP1A2 mRNAs, whereas they up-regulated several genes commonly expressed by various lineages of BM resident cells. BM-infiltrating NB cells expressed indeed the proteins encoded by the top-ranked genes, S100A8 and A9 (calprotectin), CD177 and CD3, and secreted the CXCL7 chemokine. BM-infiltrating NB cells also expressed CD271 and HLA-G. We have identified proteins specifically expressed by BM-infiltrating NB cells. Among them, calprotectin, a potent inflammatory protein, and HLA-G, endowed with tolerogenic properties facilitating tumor escape from host immune response, may represent novel biomarkers and/or targets for therapeutic intervention in high-risk NB patients.

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Microarray analysis of CaSki cell line grown as spheroids and adherent conditions

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