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Transcriptomic response of Anoxybacillus sp. SK 3-4 to aluminum exposure

ABSTRACT: By comparing the gene expression profiling in Anoxybacillus sp. SK 3-4 with and without aluminum exposure, the sets of gene up-regulated and down-regulated by aluminum were identified. The function of genes or proteins induced under these conditions can a reflection of the mechanism of resistance. Transcriptome profiling of Anoxybacillus sp. SK 3-4 treated by aluminum would allow a better understanding of the gene involving in tolerance and removal of aluminum. Global transcriptomic response of Anoxybacillus sp. SK 3-4 to aluminum exposure

ORGANISM(S): Anoxybacillus sp. SK3-4

SUBMITTER: Chun Shiong Chong

PROVIDER: E-GEOD-78908 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcriptomic response of Anoxybacillus sp. SK 3-4 to aluminum exposure

Project description:By comparing the gene expression profiling in Anoxybacillus sp. SK 3-4 with and without aluminum exposure, the sets of gene up-regulated and down-regulated by aluminum were identified. The function of genes or proteins induced under these conditions can a reflection of the mechanism of resistance. Transcriptome profiling of Anoxybacillus sp. SK 3-4 treated by aluminum would allow a better understanding of the gene involving in tolerance and removal of aluminum.

2016-08-05 | GSE78908 | GEO

Methyltioadenosine regulates liver cells proteome and methylproteome. Implications in liver biology and disease.

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2016-02-01 | PXD002957 | Pride

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2019-05-06 | PXD010643 | Pride

RNAseq of Sinorhizobium meliloti 1021 treated with the chemically synthesized peptide DgDef1 delta SP delta CTPP (representing a truncated variant of the Defensin-like peptide DgDef1 from the actinorhizal plant Datisca glomerata) against an untreated control

Project description:Plant-derived nodule-specific Cys-rich peptides (NCRs) play essential roles towards microsymbionts control. To pinpoint whether DgDef1 — a nodule-specific defensin from the actinorhizal plant Datisca glomerata — fulfills similar functions, the peptide was characterized. DgDef1 affects growth of several bacteria including Sinorhizobium meliloti. In this experiment, it was analyzed if DgDef1 without its CTPP domain (DgDef1delta SP delta CTPP) triggers a cellular response in S. meliloti. The S. meliloti strain Rm1021 was challenged in 10 mL TY medium (OD600 = 0.5) with 25 µg/mL of DgDef1 delta SP delta CTPP at 30°C, 150 rpm for 1h. The negative control was treated with acetonitrile:water (1:3) instead of DgDef1 delta SP delta CTPP. Assays were performed in four replicates. RNA was isolated and material from biological replicates were combined in equal amounts. This resulted in two RNA pools representing DgDef1 treated and untreated Rm1021 cells. The pooled RNAs were used for RNAseq in order to identify differentially expressed genes.

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Transcription profiling by array of human neuroblastoma cells treated with microRNAs 10a and 10b

Project description:Effect of microRNAs 10a and 10b on gene expression in neuroblastoma SK-N-BE cells

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Proteomics unveil a central role for peroxisomes in butyrate assimilation in the heterotrophic Chlorophyte alga Polytomella sp.

Project description:Volatile fatty acids found in effluents of the dark fermentation of biowastes can be used for mixotrophic growth of microalgae, improving productivity and reducing the cost of the feedstock. Microalgae can use the acetate in the effluents very well, but butyrate is poorly assimilated and can inhibit growth above 1 gC.L-1. The non-photosynthetic chlorophyte alga Polytomella sp. SAG 198.80 was found to be able to assimilate butyrate fast. To decipher the metabolic pathways implicated in butyrate assimilation, a large-scale differential proteomics study was developed comparing Polytomella sp. cells grown on acetate and butyrate at 1 gC.L-1.

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