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Transcription profiling of human airway epithelial cells to investigate the reversible and permanent effects of tobacco smoke exposure

ABSTRACT: RNA was obtained from histologically normal bronchial epithelium of never, former, and current smokers undergoing fiberoptic bronchoscopy. Statistical analysis of the gene expression data identified gene differentially expressed between current and never smokers and classified these genes as irreversible, slowly reversible, or rapidly reversible based on their behavior in former smokers Experiment Overall Design: Microarrays run on total RNA obtained from Bronchial Epithelium of Never Smokers (n=21), Former Smokers (n=31), and Current Smokers (n=52)

ORGANISM(S): Homo sapiens

SUBMITTER: Avrum Spira

PROVIDER: E-GEOD-7895 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Reversible and permanent effects of tobacco smoke exposure on airway epithelial gene expression.

Beane Jennifer J Sebastiani Paola P Liu Gang G Brody Jerome S JS Lenburg Marc E ME Spira Avrum A

Genome biology 20070101 9

<h4>Background</h4>Tobacco use remains the leading preventable cause of death in the US. The risk of dying from smoking-related diseases remains elevated for former smokers years after quitting. The identification of irreversible effects of tobacco smoke on airway gene expression may provide insights into the causes of this elevated risk.<h4>Results</h4>Using oligonucleotide microarrays, we measured gene expression in large airway epithelial cells obtained via bronchoscopy from never, current, a ...[more]

PMID: 17894889

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