Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Transcriptional profile of C. elegans fed E. coli (OP50) and B. subtilis (PY79)


ABSTRACT: We used RNA-seq to assay gene expression changes over time in response to OP50 and PY79 To understand the molecular processes underlying aging, we screened modENCODE ChIP-seq data to identify transcription factors that bind to age-regulated genes in C. elegans. The most significant hit was the GATA transcription factor encoded by elt-2, which is responsible for inducing expression of intestinal genes during embryogenesis. Expression of ELT-2 decreases during aging, beginning in middle age. We identified genes regulated by ELT-2 in the intestine during embryogenesis, and then showed that these developmental genes markedly decrease in expression as worms grow old. Overexpression of elt-2 extends lifespan and slows the rate of gene expression changes that occur during normal aging. Thus, our results identify the developmental regulator ELT-2 as a major driver of normal aging in C. elegans. Whole-worm mRNA was sequenced from E. coli- and B.subtilis-fed worms. For each condidtion, one replicate was sequenced at Day 4 and Day 13

ORGANISM(S): Caenorhabditis elegans

SUBMITTER: Stuart Kim 

PROVIDER: E-GEOD-79415 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Similar Datasets

2016-03-22 | E-GEOD-69263 | biostudies-arrayexpress
2016-03-22 | E-GEOD-69262 | biostudies-arrayexpress
2016-03-22 | E-GEOD-69261 | biostudies-arrayexpress
2016-03-22 | GSE79415 | GEO
2016-03-30 | E-GEOD-71720 | biostudies-arrayexpress
2012-10-11 | E-GEOD-41486 | biostudies-arrayexpress
2008-06-14 | E-GEOD-6547 | biostudies-arrayexpress
2016-03-22 | E-GEOD-69264 | biostudies-arrayexpress
2012-10-11 | GSE41486 | GEO
2019-12-31 | GSE111797 | GEO