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TGFÎ² signaling directs serrated adenomas to the mesenchymal colorectal cancer subtype [organoids]

ABSTRACT: The aim of this study was to determine the effects of TGFÎ² at the premalignant stage of CRC development. Organoid cultures were isolated from normal colon and from tubular adenomas. One normal colon culture was genetically engineered using the CRISPR-Cas9 system to carry the BRAFV600E mutation. Organoid cultures of three different tubular adenomas and two normal colon samples, and one BRAFV600E-mutant organoid culture received TGFÎ² or control treatment after which gene expression was analyzed by microarray (Affymetrix U133+ PM Genechip Array).

ORGANISM(S): Homo sapiens

SUBMITTER: Sander Hooff

PROVIDER: E-GEOD-79461 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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TGFÎ² signaling directs serrated adenomas to the mesenchymal colorectal cancer subtype [adenomas]

Project description:Colorectal cancer can be divided into four consensus molecular subtypes, which might associate with distinct precursor lesions. The aim of this study was to determine the subtype affiliation of two types of colorectal adenomas: tubular adenomas (TAs) and sessile serrated adenomas (SSAs) and to determine the activity of TGFÎ² signaling and the role of this cytokine in subtype affiliation. Adenoma samples were collected in the Academic Medical Center (AMC), Amsterdam, The Netherlands. Tubular adenomas (TAs) were obtained from familial adenomatous polyposis (FAP) patients and sessile serrated adenomas (SSAs) were collected from serrated polyposis syndrome (SPS) patients. Gene expression was analyzed for 7 sessile serrated adenomas (SSA) and 9 tubular adenomas (TA).

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TGFÎ² signaling directs serrated adenomas to the mesenchymal colorectal cancer subtype

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

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2014-09-08 | E-GEOD-48684 | biostudies-arrayexpress