Unknown,Transcriptomics,Genomics,Proteomics

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Transcriptional responses in Arabidopsis seedlings after hydrogen peroxide treatment


ABSTRACT: Excessive levels of reactive oxygen species (ROS) cause cellular stress through damage to all classes of macromolecules and result in cell death. However, ROS can also act as signaling molecules in various biological processes. In plants, ROS signaling has been documented in environmental stress perception, plant development and cell death amongst others. Knowledge on the regulatory events governing ROS signal transduction is however still scratching the surface. To further elucidate the transcriptional response and regulation upon ROS accumulation we supplemented Arabidopsis seedlings with a 10mM hydrogen peroxide (H2O2) solution to trigger oxidative stress. After growth of 7 days, hydrogen peroxide (H2O2) was added to a final concentration of 10mM. Control plants were treated with the same volume of H2O. Seedlings were grown for 24h under the same controlled conditions. Design: 3 replicates x 2 conditions (7+1 day H2O or 7+1 day H2O2)

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Patrick Willems 

PROVIDER: E-GEOD-80200 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

The ROS Wheel: Refining ROS Transcriptional Footprints.

Willems Patrick P   Mhamdi Amna A   Stael Simon S   Storme Veronique V   Kerchev Pavel P   Noctor Graham G   Gevaert Kris K   Van Breusegem Frank F  

Plant physiology 20160531 3


In the last decade, microarray studies have delivered extensive inventories of transcriptome-wide changes in messenger RNA levels provoked by various types of oxidative stress in Arabidopsis (Arabidopsis thaliana). Previous cross-study comparisons indicated how different types of reactive oxygen species (ROS) and their subcellular accumulation sites are able to reshape the transcriptome in specific manners. However, these analyses often employed simplistic statistical frameworks that are not com  ...[more]

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