Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Genome-wide mapping of HIF-2α binding under normoxia in human bronchial epithelial cells (BEAS-2B)

ABSTRACT: Growing number of cancer (stem) cells and stem cells were described to accommodate constituent active HIF-2α under normoxia. Previous study of hypoxia effect may have obscured some of normoxic HIF-2α functions as hypoxia inducible features. Our interest in study of protective potential of HIFs in lung cells led us to discover pseudohypoxia functions of HIF-2α under normoxia in human bronchial epithelial cells which exhibit pluripotency related markers and features. Our study provided perspective to pseudohypoxia functions of HIF-2α via enrichment of de novo motifs. In addition to the C-TAD, the N-TAD of HIF-2α was found to contribute to targeting on these non-canonical loci. Further elucidation of pseudohypoxia mechanism could resolve its implication of malignancy or pluripotency. We report globally mapped binding of HIF-2α under normoxia by using high throughput sequencing

ORGANISM(S): Homo sapiens

SUBMITTER: Meng-Chang Lee 

PROVIDER: E-GEOD-81635 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Json Xml
altmetric image

Publications

Genome-wide analysis of HIF-2α chromatin binding sites under normoxia in human bronchial epithelial cells (BEAS-2B) suggests its diverse functions.

Lee Meng-Chang MC   Huang Hsin-Ju HJ   Chang Tzu-Hao TH   Huang Hsieh-Chou HC   Hsieh Shen-Yuan SY   Chen Yi-Siou YS   Chou Wei-Yuan WY   Chiang Chiao-Hsi CH   Lai Ching-Huang CH   Shiau Chia-Yang CY  

Scientific reports 20160704

Constitutive functional HIF-2α was recently identified in cancer and stem cell lines under normoxia. In this study, BEAS-2B, a bronchial epithelial cell line, was shown to constitutively express active HIF-2α under normoxia and exhibit markers of pluripotency including Oct-4, Nanog, and sphere formation. Oct-4 expression was reduced after knockdown of HIF-2α under normoxia. Global enrichment analysis of HIF-2α demonstrated the diverse functions of HIF-2α under normoxia. Bioinformatics analysis o  ...[more]

Similar Datasets

Genomics Genome-wide mapping of HIF-2α binding under normoxia in human bronchial epithelial cells (BEAS-2B)
2016-07-06 | GSE81635 | GEO
Transcriptomics TET1 regulates hypoxia-induced epithelial-mesenchymal transition through DNA demethylation (hMeDIP-Seq)
2014-08-01 | E-GEOD-59989 | biostudies-arrayexpress
Transcriptomics TET1 regulates hypoxia-induced epithelial-mesenchymal transition through DNA demethylation (RNA-Seq)
2014-08-01 | E-GEOD-59987 | biostudies-arrayexpress
Transcriptomics Expression data from Hif 2alpha Knockdown study
2010-07-29 | E-GEOD-22575 | biostudies-arrayexpress
Transcriptomics JMJD2B knockdown treatment in normoxia and hypoxia
2010-09-01 | E-GEOD-18384 | biostudies-arrayexpress
Transcriptomics JMJD2B knockdown treatment in normoxia and hypoxia
2010-09-01 | GSE18384 | GEO
Transcriptomics Identification of hypoxia regulated HIF-1A and HIF-2A specific target genes in Glioblastoma
2012-02-25 | E-GEOD-27523 | biostudies-arrayexpress
Transcriptomics Effect of NDRG3 or HIF-1α knockdown on cell response to hypoxia
2015-07-24 | E-GEOD-55212 | biostudies-arrayexpress
Transcriptomics Effect of NDRG3 expression on cell response to hypoxia
2015-07-24 | E-GEOD-59729 | biostudies-arrayexpress
Transcriptomics Transcription profiling of mouse embryonic fibroblasts from embryos with ?CH1 mutation in p300 and/or CBP atreated with hypoxia or dipyridyl (a hypoxia mimetic) and trichostatin A (a histone deacetylase inhibitor) reveals two transactivation mechanisms are responsible for the bulk of HIF-1alpha-responsive gene expression
2008-06-12 | E-GEOD-3318 | biostudies-arrayexpress