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Transcription profiling of Drosophila heads heads from adult males, w118 strain

ABSTRACT: The w1118 strain is an inbred lab stock widely used in genetic and transgenic studies. w1118 males have a median life span of 35 days. Adult males were collected within 24 hr after eclosion. Approximately 200 flies were maintained in constant darkness in each food bottle at 25°C and 70% humidity and were transferred to fresh bottles every 3-4 days. Transcripts were harvested at d3 and d47. To separate the head from the rest of the body, flies were frozen and briefly vortexed in liquid nitrogen. Fly heads were collected using a sieve which retained fly bodies. Total RNA was extracted using Trizol (GIBCO/BRL). Poly(A) RNA was isolated using Oligotex resin (Qiagen). Samples were profiled with Affymetrix DrosGenome1 GeneChips, using standard Affymetrix protocol.

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Sige Zou 

PROVIDER: E-GEOD-826 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Comparing genomic expression patterns across species identifies shared transcriptional profile in aging.

McCarroll Steven A SA   Murphy Coleen T CT   Zou Sige S   Pletcher Scott D SD   Chin Chen-Shan CS   Jan Yuh Nung YN   Kenyon Cynthia C   Bargmann Cornelia I CI   Li Hao H  

Nature genetics 20040118 2

We developed a method for systematically comparing gene expression patterns across organisms using genome-wide comparative analysis of DNA microarray experiments. We identified analogous gene expression programs comprising shared patterns of regulation across orthologous genes. Biological features of these patterns could be identified as highly conserved subpatterns that correspond to Gene Ontology categories. Here, we demonstrate these methods by analyzing a specific biological process, aging,  ...[more]

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