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Transcription profiling of laminin binding and laminin non-binding germ cells to define the spermatogonial stem cells.

ABSTRACT: Comparison of laminin binding and laminin non-binding germ cells.

ORGANISM(S): Mus musculus

SUBMITTER: Nikolaus Schultz

PROVIDER: E-GEOD-829 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Defining the spermatogonial stem cell.

Hamra F Kent FK Schultz Nikolaus N Chapman Karen M KM Grellhesl Dana M DM Cronkhite Jennifer T JT Hammer Robert E RE Garbers David L DL

Developmental biology 20040501 2

Through the use of donor cells from transgenic rats expressing GFP exclusively in the germline, we have defined culture conditions where male germ cells lose (on STO cells) or maintain (on MSC-1 cells) stem cell activity. A cadre of germ cell transcripts strikingly decrease in relative abundance as a function of testis age or culture time on STO cells, but only a subset of these transcripts (approximately 248) remain elevated when cultured on MSC-1 cells. If specific gene expression regulates st ...[more]

PMID: 15110708

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