Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of Saccharomyces cerevisiae reveals Stb3 deletion affects gene expression within 10 minutes of glucose addition


ABSTRACT: Microarrays were conducted to asses the effect of Stb3 deletion in immediate transcriptional induction in response to glucose Experiment Overall Design: Response of Stb3 delete cells was compared to that of wild type (BY4742) in cells grown for 3 days in YPD and cells that received 2% glucose for 10 minutes after the 3 day growth period. Two repetitive samples were done for each timepoint.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Warren Heideman 

PROVIDER: E-GEOD-8379 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Stb3 binds to ribosomal RNA processing element motifs that control transcriptional responses to growth in Saccharomyces cerevisiae.

Liko Dritan D   Slattery Matthew G MG   Heideman Warren W  

The Journal of biological chemistry 20070706 36


Transfer of quiescent Saccharomyces cerevisiae cells to fresh medium rapidly induces hundreds of genes needed for growth. A large subset of these genes is regulated via a DNA sequence motif known as the ribosomal RNA processing element (RRPE). However, no RRPE-binding proteins have been identified. We screened a panel of 6144 glutathione S-transferase-open reading frame fusions for RRPE-binding proteins and identified Stb3 as a specific RRPE-binding protein, both in vitro and in vivo. Chromatin  ...[more]

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