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Expression data of Brain CD11b+ cells from WT and DP1 knockout mice infected with rJ2.2

ABSTRACT: PGD2 is the most abundant prostaglandin in the brain. PGD2/DP1 signaling thus has a mulitple roles in regulating the CNS immune response. We used 4 mice in each group.

ORGANISM(S): Mus musculus

SUBMITTER: Rahul Vijay

PROVIDER: E-GEOD-84709 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Expression data of Brain CD11b+ cells from WT and DP1I knockout mice

Project description:PGD2 is the most abundant prostaglandin in the brain. PGD2/DP1 signaling thus has a mulitple roles in regulating the CNS immune response. We used 4 mice in each group.

2016-07-22 | E-GEOD-84653 | biostudies-arrayexpress

Expression data of Brain CD11b+ cells from WT and DP1 knockout mice infected with rJ2.2

Project description:PGD2 is the most abundant prostaglandin in the brain. PGD2/DP1 signaling thus has a mulitple roles in regulating the CNS immune response.

2016-07-23 | GSE84709 | GEO

Expression data of Brain CD11b+ cells from WT and DP1I knockout mice

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2016-07-22 | GSE84653 | GEO

Expression data of Brain CD45+ cells from WT and STI knockout mice after WNV infection

Project description:West Nile virus (WNV) is the most important cause of endemic encephalitis in the USA. Strikingly, only a small percentage of patients develop clinical disease and of these patients, approximately 1 out of 150 patients develops encephalitis. The basis for this great variability in disease outcome is unknown, but may be related to the innate immune response. Innate immune responses, critical for control of WNV infection, are initiated by signaling through pathogen recognition receptors (PRR) such as RIG-I and MDA5. IPS-1 is a key adaptor in generating a PRR-dependent interferon response.. Here we show that IPS-1 deficiency in hematopoietic cells resulted in increased mortality and delayed WNV clearance from the brain. In IPS-1-/- mice, a dysregulated immune response was detected, characterized by a massive influx of macrophages and virus-specific T cells into the infected brain. These T cells were multifunctional and were able to lyse peptide-pulsed target cells in vitro. However, virus-specific T cells in the infected IPS-1-/- brain exhibited lower functional avidity than those in C57BL/6 brains, possibly contributing to less efficient virus clearance. The presence of virus-specific memory T cells was also not protective. We also show that macrophages were increased in numbers in the IPS-1-/- brain. Both macrophages and microglia exhibited an activated phenotype. Microarray analyses showed the preferential upregulation of genes associated with leukocyte activation and inflammation. Together, these results demonstrate the critical role that hematopoietic cell expression of Type 1 interferon and other IPS-1-dependent molecules have in WNV clearance and in regulating the inflammatory response. We used 4 mice in each group. STI knockout mice developed lethath encephalitis after WNV infectin. WT mice which have mild disease after WNV were used as controls.

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Effects of group III phospholipase A2 deficiency in mast cells on fibroblast-directed maturation of mast cells in vitro

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ChIP-seq using a Dp1 antibody in Drosophila S2R+ cells

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Pasteurella multocida strain:DP1

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RTNs and DP1 Knockdown in neurons

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2019-02-15 | PXD012264 | Pride

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