Unknown,Transcriptomics,Genomics,Proteomics

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SiRNA of mouse undifferentiated Dicer -/- (27H10) embryonic stem cells


ABSTRACT: We have analyzed the transcript expression levels in Dicer knock-out embryonic stem (ES) cells 24 hours after transfection with either control siRNA agains Renilla luciferase or miRNA Mimics (Dharmacon) of mmu-miR-290 cluster members in order to identify primary targets of miR-290 cluster miRNAs. Experiment Overall Design: Cell were analysed 24h after transfections in an undifferentiated state. Triplicates of both transfections were analyzed.

ORGANISM(S): Mus musculus

SUBMITTER: Lasse Sinkkonen 

PROVIDER: E-GEOD-8503 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

MicroRNAs control de novo DNA methylation through regulation of transcriptional repressors in mouse embryonic stem cells.

Sinkkonen Lasse L   Hugenschmidt Tabea T   Berninger Philipp P   Gaidatzis Dimos D   Mohn Fabio F   Artus-Revel Caroline G CG   Zavolan Mihaela M   Svoboda Petr P   Filipowicz Witold W  

Nature structural & molecular biology 20080302 3


Loss of microRNA (miRNA) pathway components negatively affects differentiation of embryonic stem (ES) cells, but the underlying molecular mechanisms remain poorly defined. Here we characterize changes in mouse ES cells lacking Dicer (Dicer1). Transcriptome analysis of Dicer-/- cells indicates that the ES-specific miR-290 cluster has an important regulatory function in undifferentiated ES cells. Consistently, many of the defects in Dicer-deficient cells can be reversed by transfection with miR-29  ...[more]

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