Genome-wide transcriptome analyses by RNAseq to determine the change that happens in the peripheral taste system with age.
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ABSTRACT: To understand the mechanisms that regulate the renewal and maintenance of taste cells we performed RNA-sequencing analysis on isolated taste cells from 2 and 6 month old mice to determine how alterations in the taste cell-transcriptome regulate taste cell maintenance and function in adults. We found that the Activator Protein-1 (AP1) transcription factors (c-Fos, Fosb and c-Jun) and genes associated with this pathway were significantly downregulated in taste cells from 6 month old mice and further declined at 12 months. We generated conditional c-Fos- knockout mice to target K14-expressing cells, including differentiating taste cells. c-Fos deletion caused a severe perturbation in taste bud structure and resulted in a significant reduction in the taste bud size. c-Fos deletion also affected taste cell turnover as evident by, a decrease in proliferative markers, and upregulation of the apoptotic marker cleaved-PARP. Thus, AP1 factors are important regulators of adult taste cell renewal and their downregulation negatively impacts taste maintenance. Comparison of genes responsible for peripheral Taste system maintenance at age, 3 and 6 months. processed_data.txt: List of B vs S gene expression data gene_exp.diff: cuffdiff data for 2 and 6 month taste cells
ORGANISM(S): Mus musculus
SUBMITTER: Jayasha Shandilya
PROVIDER: E-GEOD-85308 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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