Unknown,Transcriptomics,Genomics,Proteomics

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Effect pck or ppc expression on intracellular ATP


ABSTRACT: Up-regulation of motility genes and biosynthesis genes were found in the pck expressing high ATP cell by transcriptome analysis while catabolism genes were down-regulated. Keywords: response depends on intracellular ATP concentration derived by pck or ppc overexpression 1. pck or ppc overexpressing E. coli at early log phase using glucose-minimal medium 2. pck or ppc overexpressing E. coli at chemostat culture (D=0.1 h-1)using LB-glucose medium

ORGANISM(S): Escherichia coli

SUBMITTER: Pil Kim 

PROVIDER: E-GEOD-8532 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

A physiology study of Escherichia coli overexpressing phosphoenolpyruvate carboxykinase.

Kwon Yeong-Deok YD   Lee Sang Yup SY   Kim Pil P  

Bioscience, biotechnology, and biochemistry 20080407 4


To determine whether intracellular ATP levels can be affected, Escherichia coli overexpressing phosphoenolpyruvate carboxykinase (pck) or phosphoenolpyruvate carboxylase (ppc) were grown in glucose minimal medium. The Pck-overexpressing cells showed approximately twice the intracellular ATP concentration, with 22% slower growth than the Ppc-overexpressing strain. This unexpected result of higher ATP coupled with slower growth is discussed based on transcriptome analysis. ...[more]

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