Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

57 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Della-regulation of salt stress responses-DELLAs contribute to salt stress responses

ABSTRACT: rs06-07_della - della-regulation of salt stress responses - Identification of DELLA-dependent dowtream targets in response to salt stress - Aim was to determine downstream target of DELLA proteins involved in salt stress tolerance. Wt, ga1-3, penta seeds were sterilized, sown on MS agar plates then put for stratification for 3 days at 4degreeC. Plates were placed in growth cabinet for 9 days. Seedlings were transferred to 24 well-plates, with 2 seedlings per well (0.5 ml MS liquid per well). Plates were placed in the same condition for 3 days. Finally, NaCl were added (final concentration 200 mM), except for the control. The salt treatment was applied for 30 min and 1h. Treatment was stopped by freezing in liquid nitrogen. Keywords: dose response,gene knock out,time course 12 dye-swap - CATMA arrays

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Jean-Pierre Renou

PROVIDER: E-GEOD-8556 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Biodiversity and functional genomics of endogenous small RNAs induced upon biotic and abiotic stresses in plant (gemsup)

Project description:au07-12_geminiselsup - gemsup - Determine the level of expresison in transgenic plants expressing geminviral gene silencing suppressors. - Transgenic lines of Arabidopsis overexpressing three different geminiviral silencing suppressors: C2, C4 and V2 were grown in adition to a control and their total RNA was extracted in order to study their genetic expressions profiles. Keywords: gene knock in (transgenic) 9 dye-swap - CATMA arrays

2010-10-12 | E-GEOD-24475 | biostudies-arrayexpress

Transcriptomic of meiocytes.

Project description:What are the gene expressed during meiosis pathway in Arabidopsis thalina. Comparison of total RNAs from isolated meiocytes with those isolated from root tips, and those from leaves. Comparison of transcriptomes of normal stamen (ecotype columbia) with root tips and leaves. And, comparison of transcriptomes of normal stamens (ecotype L. erecta) with stamens from sporocyteless mutant (L. erecta). Keywords: organ comparison 11 dye-swap - CATMA arrays

2010-05-15 | E-GEOD-10229 | biostudies-arrayexpress

Infection with Salmonella or Pseudomonas or E. coli - Interactions between plants and human pathogenic bacteria.

Project description:au07-07_salmonella - infection with Salmonella or Pseudomonas or E. coli. Identification of genes involved in early Arabidopsis response to pathogenic and non-pathogenic bacteria. Arabidopsis thaliana Col-0 seedlings were infected for 2 hours with a) Salmonella typhimurium strain 14028s, b) Pseudomonas syringae DC3000 or c) Escherichia coli DH5A Keywords: treated vs untreated comparison 6 dye-swap - CATMA arrays

2010-06-05 | E-GEOD-20996 | biostudies-arrayexpress

fe-fit-diff_6d-Differential gene expression in A. thaliana roots depending on iron supply and FIT (FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR) abundance

Project description:au15-01_iron-fit - fe-fit-diff_6d - Changes in gene expression profiles between fit knock-out, wild-type and FIT overexpressor seedlings under sufficient iron supply and under iron deficiency. - Col-0, HA-FIT and fit-3. 21 dye-swap - gene knock in (transgenic),gene knock out,treated vs untreated comparison

2016-07-01 | E-GEOD-80281 | biostudies-arrayexpress

aba4/1-Analyse of arabidopsis mutants and transforming affected in the synthesis of abscissic acid.

Project description:ra07-03_aba4 - aba4/1 - Identification of genes deregulated in aba4 mutant or overexpressers. - Comparison of different genotypes to wild type Col-0: aba4-3 mutant, two independent 35S-ABA4 transgenic lines and two independent 35S-NCED6 transgenic lines. Keywords: gene knock in (transgenic),gene knock out 6 dye-swap - CATMA arrays

2008-05-20 | E-GEOD-10357 | biostudies-arrayexpress

Gene expression of lpp2 KO mutant in response to ABA.

Project description:Transcriptomic study of lpp2 KO mutant in response to abcsisic acid - Fully developed rosette leaves were immersed for 1 min in a solution of 10-5 M ABA with 0.1% DMSO and 0.05%silwet. RNA are extracted after a 3h incubation. This treatment is compared to control (1 min immersion in 0.1% DMSO and 0.05%silwet) then 3h incubation. Keywords: treated vs untreated comparison 4 dye-swap - CATMA arrays

2010-05-16 | E-GEOD-14446 | biostudies-arrayexpress

wt/myb77-Functional characterization of AtMYB77 transcription factor.

Project description:au07-03_myb77 - wt/myb77 - Transcriptome analysis of Myb77 knock out mutant line or of Myb77fragment overxpressing transgenic line. - Plants were grown in vitro on 1/2 MS medium, 3% sucrose, 0.8% agar for 21 days under balance day cycle (12 hours daylight at 22Â°C; 12 hours dark at 20Â°C) in climate chamber. The whole plants were collected for RNAs extraction. Keywords: gene knock in (transgenic),gene knock out 4 dye-swap - CATMA arrays

2010-05-25 | E-GEOD-10673 | biostudies-arrayexpress

fwa-pollen-Effect of DNA hypomethylation on gene expression

Project description:au05-02_fwa - fwa-pollen - Which gene are upregulated in a hypomethylated mutant? - Compare gene expression between WT pollen and mutant pollen Keywords: gene knock out 4 dye-swap - CATMA arrays

2010-05-26 | E-GEOD-10717 | biostudies-arrayexpress

mir398 mutant analysis-Analysis of miR398 mutants.

Project description:adt07-03_mir398-mutants - mir398 mutant analysis - Defining new targets of miR398 by transcriptome analysis of two miR398 mutant alleles. - Plants were grown on soil in culture chambers under the following conditions, a day/night cycle of 16/8 hours was applied, light intensity was around 100-150 umol m-2 s-1, temperature day/night was 20/15Â°C. Plants were harvested 17 days later and RNAs were then extracted. The experiment was repeated twice. Keywords: gene knock out 4 dye-swap - CATMA arrays

2010-05-25 | E-GEOD-10672 | biostudies-arrayexpress

analysis of npc48 overexpressing plants-Analysis of npc48 overexpressing plants.

Project description:rs07-02_npc48 - analysis of npc48 overexpressing plants - Identification of potential targets of the non-protein codding RNA npc48. - We have lines overexpressing npc48 that shhowed a leaf morphological phenotype (serrated leaves). The proportion of lines havin gthe phenotype is around 20% of total transformants. We wanted to compare the transcriiptome of npc48-overexpressing lines with and without phenotype together with a transformation control for an additional control. 4 dye-swap - CATMA arrays

2008-04-12 | E-GEOD-10679 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data