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Transcription profiling of effects of brassinolide on Arabidopsis yucca mutant and wild type seedlings

ABSTRACT: Ten-day old light-grown Arabidopsis seedlings were immersed in 1 ÂµM brassinolide in one-half-strength Murashige Minimal Organics Medium (Invitrogen, Carlsbad, CA) or media alone for 2.5 hours

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Jennifer Nemhauser

PROVIDER: E-GEOD-862 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Interdependency of brassinosteroid and auxin signaling in Arabidopsis.

Nemhauser Jennifer L JL Mockler Todd C TC Chory Joanne J

PLoS biology 20040824 9

How growth regulators provoke context-specific signals is a fundamental question in developmental biology. In plants, both auxin and brassinosteroids (BRs) promote cell expansion, and it was thought that they activated this process through independent mechanisms. In this work, we describe a shared auxin:BR pathway required for seedling growth. Genetic, physiological, and genomic analyses demonstrate that response from one pathway requires the function of the other, and that this interdependence ...[more]

PMID: 15328536

Similar Datasets

BR effects on WT and yucca seedlings

Project description:Ten-day old light-grown Arabidopsis seedlings were immersed in 1 µM brassinolide in one-half-strength Murashige Minimal Organics Medium (Invitrogen, Carlsbad, CA) or media alone for 2.5 hours Keywords: repeat sample

2004-08-31 | GSE862 | GEO

Transcription profiling of Arabidopsis seedlings treated with auxin

Project description:Five-day old light-grown Arabidopsis seedlings were immersed 10 ÂµM indole-3-acetic acid (auxin) or water

2008-06-16 | E-GEOD-863 | biostudies-arrayexpress

Transcription profiling of Arabidopsis seedlings treated with chitosan

Project description:We treated Arabidopsis seedlings with chitosan and carried out a transcript profiling analysis (GeneChip microarrays) in order to identify genes and transcription factors regulated by chitosan. The results showed that jasmonate and defense responsive genes, camalexin and lignin biosynthetic genes were among genes up-regulated by chitosan. Several transcription factors are also strongly induced by chitosan. The results suggested that chitosan can be used as a strong elicitor of defense pathways. Experiment Overall Design: Arabidopsis thaliana, ecotype Columbia (Col-0) seeds were sterilized for 7 min in 1.7% (v/v) bleach solution, incubated over night in 4% PPM (Plant Preservative Mixture, Plant Cell Technology, Washington DC, USA) in a full strength sterilized Murashige-Skoog (MS) salt solution with gentle shaking. Subsequently, the seeds were rinsed in abundant sterile water and transferred into 2.5 ml liquid growing media (MS half strength solution) with 0.05% PPM in 6-well plates. The plates were incubated in the dark at 4 Â°C for two days and finally transferred to continuous light (90Âµm photons/ m-2) with gentle swirling for four days in a plant growth chamber at 22 Â°C. Experiment Overall Design: . Treatments were performed by comparing a control solution containing the solvent used to solubilize chitosan, and a chitosan solution concentrated 150 Âµg/ml.

2009-11-10 | E-GEOD-17193 | biostudies-arrayexpress

Transcription profiling of Arabidopsis dark grown seedlings response to 6h-long treatment with 90 mM sucrose

Project description:Dark grown Arabidopsis seedlings (Columbia gl1) were grown in the dark at 23Â°C for 4 days before adding 90 mM sucrose for 6h. Experiment Overall Design: Arabidopsis thaliana ecotype Columbia glabra (gl1-1) was used in this study. Seeds were sterilized with diluted bleach (10 min incubation in 1.7% sodium hypochlorite, rinsing and washing thoroughly in sterile water), and incubated in 2.5 ml liquid growing medium [Murashige-Skoog (MS) half strength solution] in 6-well plates (fully aerobic, with shaking). Plates were incubated in the darkness at 4Â°C for 2 days and finally transferred to 23Â°C for 4 days before the sugar treatments (with 90 mM sucrose, lasting 6h). Two independent, replicated experiments were performed for sucrose treatments. Each independent experiment consisted of four replicated seedlings cultures, pooled after RNA extraction. Experiment Overall Design: AIR EXPERIMENT 1: Control without added sucrose Experiment Overall Design: AIR EXPERIMENT 2: Control without added sucrose Experiment Overall Design: Sucrose 90 mM, 6h treatment in the dark, Biological Replicate 1 Experiment Overall Design: Sucrose 90 mM, 6h treatment in the dark, Biological Replicate 2

2008-06-12 | E-GEOD-3704 | biostudies-arrayexpress

Auxin effects on seedlings

Project description:Five-day old light-grown Arabidopsis seedlings were immersed 10 µM indole-3-acetic acid (auxin) or water Keywords: repeat sample

2004-07-31 | GSE863 | GEO

RNA-seq of roots of Schrenkiella parvula seedlings treated with 0mM or 175mM NaCl for 0 h, 3 h, 24 h and 48 h.

Project description:The purpose of the experiments is to understand transcriptional reprogramming in roots of halophyte Schrenkiella parvula (S. parvula) that may contribute to its high salt tolerance. Root materials were harvested from S. parvula seedlings grown on agar plates with ½ strength Murashige-Skoog (½MS) medium including vitamins for 4 days and transferred to ½MS medium supplemented with 0mM or 175mM NaCl for 0h, 3h, 24h or 48h.

2023-02-18 | E-MTAB-12424 | biostudies-arrayexpress

Time course of brassinolide treatment on Arabidopsis seedlings

Project description:Time course of brassinolide treatment on Arabidopsis seedlings

| PRJNA615658 | ENA

Time course of brassinolide treatment on Arabidopsis seedlings

Project description:Brassinosterioids (BRs) are plant hormones known to regulate many processes such as growth and development. However, how plants respond termporally BR signaling is not as well understaood. Thus, the goal of this study was to determine the temporal transcriptional changes in Arabidopsis seedlings treated with brassinolide. We determined differentially expressed genes at each time point to see how the plant response to BR evolves over time.

2021-08-23 | GSE147589 | GEO

Transcription profiling of Arabidopdis seedlings - heat, anoxia, and combined heat-anoxia treatments

Project description:Anoxia induces several heat shock proteins and a heat pre-treatment can acclimatize Arabidopsis seedlings to a subsequent anoxic treatment. In this work we analyzed the response of Arabidopsis seedlings to anoxia, heat and a combined heat+anoxia stress. A significant overlapping between the anoxic and heat shock responses has been observed by whole-genome microarray analysis. Experiment Overall Design: We treated Arabidopsis seedling, 4-days old, dark germinated with: Experiment Overall Design: -Control (23Â°C, dark, liquid Murashige-Skoog medium containing 30mM sucrose). Experiment Overall Design: -Heat-treated (38Â°C for 90 minutes, dark, liquid Murashige-Skoog medium containing 30mM sucrose). Experiment Overall Design: -Anoxia-treated (23Â°C, under anoxia for 6h, dark, liquid Murashige-Skoog medium containing 30mM sucrose). Experiment Overall Design: -combined heat+Anoxia-treatment (23Â°C, treated at 38Â°C for 90 min and thereafter under anoxia for 6h, dark, liquid Murashige-Skoog medium containing 30mM sucrose). Experiment Overall Design: Two biological replicates for each condition.

2009-06-08 | E-GEOD-16222 | biostudies-arrayexpress

Mild proteasomal stress improves photosynthetic performance in Arabidopsis chloroplasts

Project description:The ppi2 mutant introgressed into the Columbia-0 ecotype as previously reported. Doublemutant ppi2xrpn8a was crossed with the above referenced genotypes using heterozygous ppi2 plants. After 2 days of stratification at 4 °C the plants were grown on half-strength Murashige and Skoog (M&S) medium supplemented with 0.8% (w/v) plant agar (Duchefa) and 0.8% (w/v) sucrose under short day conditions.

2020-05-27 | PXD017126 | Pride

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