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Transcription profiling of Arabidopsis exposed to glyphosate herbicide treatment


ABSTRACT: Using whole genome microarray (Affymetrix ATH1) we studied the transcriptional response of Arabidopsis thaliana to glyphosate (Roundup Original) herbicde that inhibits 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) enzyme and thus disrupts aromaticamino acid biosynthesis. Few genes related to defense and secondary metabolism were altered. Experiment Overall Design: Surfactant (preference 0.25%) treated plants were used as carrier control group and EC50 concentration of glyphosate was used as the herbicide treatment group. Each of the control and treatment group consisted of 3 biological replicates and each biological replicates comprised leaves from 10 individual plants. RNA was extracted at 24h post treatment to study the transcriptional alterations caused by the herbicide treatment.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Malay Das 

PROVIDER: E-GEOD-8927 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

A composite transcriptional signature differentiates responses towards closely related herbicides in Arabidopsis thaliana and Brassica napus.

Das Malay M   Reichman Jay R JR   Haberer Georg G   Welzl Gerhard G   Aceituno Felipe F FF   Mader Michael T MT   Watrud Lidia S LS   Pfleeger Thomas G TG   Gutiérrez Rodrigo A RA   Schäffner Anton R AR   Olszyk David M DM  

Plant molecular biology 20091231 4-5


In this study, genome-wide expression profiling based on Affymetrix ATH1 arrays was used to identify discriminating responses of Arabidopsis thaliana to five herbicides, which contain active ingredients targeting two different branches of amino acid biosynthesis. One herbicide contained glyphosate, which targets 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS), while the other four herbicides contain different acetolactate synthase (ALS) inhibiting compounds. In contrast to the herbicide cont  ...[more]

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