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Transcription profiling of rat glomeruli: conventionally-isolated and laser capture microdissected glomeruli

ABSTRACT: We compared the gene expression analysis of 2 different glomerular isolation techniques (laser capture microdissection with 2 rounds of RNA amplification and unamplified glomerular RNA after iron perfusion with glomerular sieving) and obtained different results depending on the glomerular isolation technique that was used Experiment Overall Design: Paired kidneys from Lewis-Hsd rat kidneys were collected. The glomeruli of one kidney was obtained by LCM with 2 rounds of RNA amplification while the unamplified glomerular RNA of the contralateral kidney was obtained after perfusion with iron oxide and isolated by magnetic purification and a glomerular sieving technique. The RNA was analyzed using the Affymetrix Rat 230A microarrays.

ORGANISM(S): Rattus norvegicus

SUBMITTER: Jian Zhou

PROVIDER: E-GEOD-8998 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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