Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Transcription profiling of human umbilicial vein endothelial cells after TNF-alpha treatment

ABSTRACT: The many steps involved in the production of a mature mammalian mRNA are extensively coupled, and levels of both precursors and products can be measured using expression and genomic tiling microarrays. Different probes in these arrays targeting the same transcript often give different signals; then, precursor (nascent) RNA – which is present transiently at low concentrations – is difficult to detect. Experiment Overall Design: Using tumor necrosis factor a (TNF-alpha) to switch on transcription of selected human genes rapidly and synchronously, we apply samples collected every 15 min to arrays

ORGANISM(S): Homo sapiens

SUBMITTER: Shuichi Tsutsumi 

PROVIDER: E-GEOD-9055 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Json Xml
altmetric image

Publications

Genome-wide studies reveal that transcription by RNA polymerase II (Pol II) is dynamically regulated. To obtain a comprehensive view of a single transcription cycle, we switched on transcription of five long human genes (>100 kbp) with tumor necrosis factor-alpha (TNFalpha) and monitored (using microarrays, RNA fluorescence in situ hybridization, and chromatin immunoprecipitation) the appearance of nascent RNA, changes in binding of Pol II and two insulators (the cohesin subunit RAD21 and the CC  ...[more]

Similar Datasets

Transcriptomics Global expression analysis of HNF4alpha and HNFgamma-mediated transcriptional control
2010-12-22 | E-GEOD-18973 | biostudies-arrayexpress
Transcriptomics Expression data of HUVEC cells after statin treatment
2013-01-01 | E-GEOD-32547 | biostudies-arrayexpress
Transcriptomics Expression data of statin treated HUVEC cells transfected siRNA KLF2 or KLF4.
2013-01-01 | E-GEOD-32693 | biostudies-arrayexpress
Transcriptomics Expression data of HUVEC cells after PPARβ/δ agonist and/or hypoxia treatment
2014-03-06 | E-GEOD-50378 | biostudies-arrayexpress
Transcriptomics Transcription profiling of human HUVECs before and after Angiopoietin stimulation
2008-06-16 | E-GEOD-9677 | biostudies-arrayexpress
Genomics Proteomic analysis of native hepatocyte nuclear factor-4{alpha} (HNF4{alpha}) isoforms, phosphorylation status, and interactive cofactors.
2010-12-22 | E-GEOD-18990 | biostudies-arrayexpress
Transcriptomics Human PBMNCs: Control vs. Patients with Kawasaki disease
2010-09-04 | E-GEOD-17975 | biostudies-arrayexpress
Transcriptomics Transcription profiling of rat liver and adipose tissues after 24 h-fasting
2008-06-15 | E-GEOD-7623 | biostudies-arrayexpress
Transcriptomics Effects of far-infrared rays to human prostate cancer cell line PC-3
2013-03-14 | E-GEOD-16077 | biostudies-arrayexpress
Transcriptomics Gene expression profiles in mouse embryonic fibroblasts (MEFs) derived from BCL11B-KO mice
2016-09-05 | E-GEOD-83980 | biostudies-arrayexpress