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Identification of Myc-regulated microRNAs: human and mouse

ABSTRACT: This SuperSeries is composed of the following subset Series: GSE9070: Identification of Myc-regulated microRNAs in human P493 cells GSE9104: Identification of Myc-regulated microRNAs in mouse B cell lymphomas Keywords: SuperSeries Refer to individual Series

ORGANISM(S): Homo sapiens

SUBMITTER: Tsung-Cheng Chang

PROVIDER: E-GEOD-9129 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Widespread microRNA repression by Myc contributes to tumorigenesis.

Chang Tsung-Cheng TC Yu Duonan D Lee Yun-Sil YS Wentzel Erik A EA Arking Dan E DE West Kristin M KM Dang Chi V CV Thomas-Tikhonenko Andrei A Mendell Joshua T JT

Nature genetics 20071209 1

The c-Myc oncogenic transcription factor (Myc) is pathologically activated in many human malignancies. Myc is known to directly upregulate a pro-tumorigenic group of microRNAs (miRNAs) known as the miR-17-92 cluster. Through the analysis of human and mouse models of B cell lymphoma, we show here that Myc regulates a much broader set of miRNAs than previously anticipated. Unexpectedly, the predominant consequence of activation of Myc is widespread repression of miRNA expression. Chromatin immunop ...[more]

PMID: 18066065

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Identification of Myc-regulated microRNAs in human P493 cells

2007-12-05 | E-GEOD-9070 | biostudies-arrayexpress

Identification of Myc-regulated microRNAs in mouse B cell lymphomas

Project description:We previously described the use of a spotted oligonucleotide array to identify the mir-17 cluster as a direct transcriptional target of Myc. In order to determine whether Myc regulates additional miRNAs, we produced custom microarrays with an expanded set of probes capable of assaying the expression of 313 human miRNAs and 233 mouse miRNAs. miRNA expression was assayed in a murine model of Myc-induced B cell lymphoma. In this system, bone marrow from p53-/- mice is infected with a retrovirus that produces a Myc-estrogen receptor fusion protein (MycER). Infected cells form polyclonal B cell lymphomas in the presence of 4-hydroxytamoxifen (4-OHT), which activates the MycER fusion protein. RNA from subcutaneous tumors with high Myc activity (animals treated continuously with 4-OHT) and low Myc activity (animals in which 4-OHT was withdrawn after tumor formation) was analyzed. Keywords: Dose Response RNA from 2 subcutaneous MycER tumors with high Myc activity (animals treated continuously with 4-OHT) and 2 tumors with low Myc activity (animals in which 4-OHT was withdrawn after tumor formation) was analyzed.

2010-05-22 | E-GEOD-9104 | biostudies-arrayexpress

Ectopic Myc expression

Project description:Ectopic Myc Expression in P493-6 B-cells at three levels: High Myc, Low Myc and No Myc High Myc, Low Myc and No Myc expressions

2010-03-09 | E-GEOD-19703 | biostudies-arrayexpress

Homo sapiens

Project description:Identification of Myc-regulated microRNAs in human P493 cells

| PRJNA105209 | ENA

Chromatin occupancy of key transcriptional regulators in P493-6 cells with various levels of c-Myc expression

Project description:P493-6 cells are immortalized human peripheral B cells that carry a conditional, tetracycline-regulated myc gene. We present ChIP-seq analysis of key transcritional regulators in P493-6 cells expressing various levels of c-Myc: 0hr (low c-Myc levels), 1hr (intermediate c-Myc levels), 24hr (very high c-Myc levels) and No Tet (steady-state c-Myc levels). Brd4, c-Myc, Max, Med1, RNAPII, and the chromatin modification H3K27Ac were profiled in P493-6 cells

2012-11-14 | E-GEOD-42262 | biostudies-arrayexpress

Chromatin occupancy of key transcriptional regulators in P493-6 cells with various levels of c-Myc expression

2012-11-14 | GSE42262 | GEO

Selective transcriptional regulation by Myc in cellular growth control and lymphomagenesis [ChIP-Seq]

Project description:The c-myc proto-oncogene product, Myc, is a transcription factor that binds thousands of genomic loci. Recent work suggested that rather than up- and down-regulating selected groups of genes, Myc targets all active promoters and enhancers in the genome (a phenomenon termed "invasion") and acts as a general amplifier of transcription. However, the available data did not readily discriminate between direct and indirect effects of Myc on RNA biogenesis. We addressed this issue with genome-wide chromatin immunoprecipitation and RNA expression profiles during B-cell lymphomagenesis in mice, in cultured B-cells and fibroblasts. Consistent with long-standing observations, we detected general increases in total RNA or mRNA copies per cell (hereby termed "amplification") when comparing actively proliferating cells with control quiescent cells: this was true whether cells were stimulated by mitogens (requiring endogenous Myc for a proliferative response) or by deregulated, oncogenic Myc activity. RNA amplification and promoter/enhancer invasion by Myc were separable phenomena that could occur without one another. Moreover, whether or not associated with RNA amplification, Myc drove the differential expression of distinct subsets of target genes. Hence, while having the potential to interact with all active/poised regulatory elements in the genome, Myc does not directly act as a global transcriptional amplifier. Instead, our results imply that Myc activates and represses transcription of discrete gene sets, leading to changes in cellular state that can in turn feed back on global RNA production and turnover. Mapping c-Myc, RNAPII and H3K4me3, H3K4me1 and H3K27ac in four different models of Myc regulation Note: GEO Sample GSM894093 was used as input sample to generate processed data files P493.Myc.NoMyc.bed, P493.Myc.LowMyc.bed, P493.Myc.HighMyc.bed, P493.Myc.t1h.bed, P493.Myc.t24h.bed, P493.Pol2.NoMyc.bed, P493.Pol2.t24h.bed

2014-07-07 | E-GEOD-51004 | biostudies-arrayexpress

Ectopic Myc expression

Project description:Ectopic Myc Expression in P493-6 B-cells at three levels: High Myc, Low Myc and No Myc

2010-02-25 | GSE19703 | GEO

MYC Targeted Long Noncoding RNA DANCR Promotes Cancer in Part by Reducing p21 Levels.

Project description:p493 RNA-seq in the MYC on and MYC off conditions

2018-09-21 | GSE120246 | GEO

Gene expression from human B-lymphocytes (P493-6)

Project description:P493-6 contain a tet repressible MYC contruct. In the presense of tetracycline, MYC levels are great reduced and the cells cease to cycle. Gene expression was compared between high and low MYC expressing cells P493-6 cells were grown in both the presence and absence of tetracycline. RNA was extracted and gene expression analysis was performed using exon arrays

2011-09-18 | E-GEOD-32219 | biostudies-arrayexpress

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