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Transcription profiling of mouse adipose tissue from Pparg animals

ABSTRACT: Identifying gene expression changes in adipose tissue of lipodystrophic Pparg targeted mice Experiment Overall Design: RNA from epididymal white adipose pads of three 10-week-old Pparg males and three litter-matched WT controls was analyzed by MOE430v2.0 GeneChipâ?¢ arrays, one mouse per array.

ORGANISM(S): Mus musculus

SUBMITTER: Yaacov Barak

PROVIDER: E-GEOD-9131 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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A mouse model of conditional lipodystrophy.

Kim Suyeon S Huang Li-Wen LW Snow Kathy J KJ Ablamunits Vitaly V Hasham Muneer G MG Young Timothy H TH Paulk Angelique C AC Richardson Joel E JE Affourtit Jason P JP Shalom-Barak Tali T Bult Carol J CJ Barak Yaacov Y

Proceedings of the National Academy of Sciences of the United States of America 20071005 42

Lipodystrophies are syndromes of adipose tissue degeneration associated with severe defects in lipid and glucose homeostasis. We report here the generation and analysis of Pparg(ldi), a targeted allele that confers conditional dominant lipodystrophy in mice. The Pparg(ldi) allele was generated by insertion of the Tet activator (tTA) and a tTA-regulated Flag-Pparg1 transgene into the Pparg gene. Unexpectedly, tTA elicits mild lipodystrophy, insulin resistance, and dyslipidemia, and the Flag-PPARg ...[more]

PMID: 17921248

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