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RNA validation of LAB 2k v2

ABSTRACT: To study their metabolic potential in natural ecosystems, we developed a species-independent LAB microarray, containing 2,269 30-mer oligonucleotides, and targeting 406 genes that play a key role in the production of sugar catabolites, bacteriocins, exopolysaccharides, and aromas, in probiotic and biosafety characteristics, and in stress response. Also, genes linked to negative traits such as antibiotic resistance and virulence are represented. This experiment is a validation experiment, where we hybridized labelled RNA from 20 LAB strains, covering 86% of all oligos. Keywords: Platform validation experiment Loop design, hence all RNA samples were hybridized once in each colour (Cy3 and Cy5).

ORGANISM(S): Lactobacillus pontis

SUBMITTER: Stefan Weckx

PROVIDER: E-GEOD-9140 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Development and validation of a species-independent functional gene microarray that targets lactic acid bacteria.

Weckx Stefan S Allemeersch Joke J Van der Meulen Roel R Vrancken Gino G Huys Geert G Huys Geert G Vandamme Peter P Van Hummelen Paul P De Vuyst Luc L

Applied and environmental microbiology 20090814 20

During the last few years, genome-related information has become available for many microorganisms, including important food-related bacteria. Lactic acid bacteria (LAB) are important industrially in the production of fermented foods such as dairy products, sausages, sourdoughs, and vegetables. Despite their limited metabolic capacity, LAB contribute considerably to important characteristics of fermented foods, such as flavor and texture. In the present study, a species-independent functional ge ...[more]

PMID: 19684161

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