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Transcription profiling of Arabidopsis 10 day old wild type and chl1-5 plants exposed to 25 mM nitrate for 0h or 0.5h

ABSTRACT: CHL1 is one of major nitrate transporters responsible for nitrate uptake and some studies suggest that it may be also play a role in regulating the nitrate response, its influence was analyzed in a global transcriptome study using Affymetrix ATH1 array. Experiment Overall Design: Three biological replicates of 10-day-old Arabidopsis root tissue from wild-type and chl1-5 plants were performed to explore differences in gene expression between the wild-type and chl1-5 mutants exposed to 25 mM nitrate for 0h (T0) or 0.5h (T0.5) using Affymetrix ATH1 microarray.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Yi-Fang Tsay

PROVIDER: E-GEOD-9148 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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CHL1 functions as a nitrate sensor and also is one of major nitrate transporters responsible for nitrate responses and uptake.

Project description:CHL1 functions as a nitrate sensor and also is one of major nitrate transporters responsible for nitrate responses and uptake. ANI is a protein phosphatase particiates in temporal nitrate response, its influence was analyzed in a global transcriptome study using Affymetrix ATH1 array. Two biological replicates of 10-day-old Arabidopsis root tissue from wild-type and ani1 plants were performed to explore differences in gene expression between the wild-type and ani1 mutants exposed to 200 μM nitrate for 0h (T0), 0.5h (T0.5), or 16h (T16) using Affymetrix ATH1 microarray.

2014-10-26 | E-GEOD-33242 | biostudies-arrayexpress

Impact of NRT1.1 point mutants on gene expression under high NH4NO3 supply

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Transcriptome analysis of Arabidopsis shoots and roots whose nitrate levels and IPT3 expression are manipulated in a shoot-specific manner

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Characterization of temporal and spatial patterns of gene expression in response to nitrate in Arabidopsis roots

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Response of Arabidopsis roots to combinations of nitrogen and hormonal signals

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2016-10-26 | E-GEOD-71737 | biostudies-arrayexpress

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Root nitrate response of wild type plants and afb3-1 mutant plants in Arabidopsis thaliana

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2012-04-30 | E-GEOD-35544 | biostudies-arrayexpress

Impact of NRT1.1 point mutants on gene expression under high NH4NO3 supply

2015-01-15 | GSE49437 | GEO

Enhanced photorespiratory and TCA pathways by elevated CO2 to manage ammonium nutrition in tomato leaves

Project description:This work aims to study wether the increment of the atmospheric carbon dioxide (CO2) concentration, in the context of climate change, will potentially allow plants to better face ammonium nutrition. Tomato (Solanum lycopersicum L.) plants were grown for 4 week with 15 mM of nitrogen, supplied as nitrate or ammonium, in conditions of ambient (aCO2, 400 ppm) or elevated CO2 (eCO2, 800 ppm) atmosphere. Transcription profiling by array was carried out in leavesfor the four growth conditions assayed and gene expression comparisons were done between N sources and CO2 conditions: i) genes differentially expressed in response to the atmospheric CO2 concentration (eCO2 vs aCO2) under nitrate or ammonium nutrition; ii) genes differentially expressed in response to the N source (ammonium vs nitrate) at aCO2 or eCO2. 3 biological replicates for each growth condition were analysed.

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Transcriptome analysis of the shoots of Arabidopsis wild-type and mutants deficient in either or both activities of nitrate reductase and alternative oxidase

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