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Transcription profiling of Drosophila body wall and wing/hinge fragments from wing imaginal discs to identify genes with localized expression patterns

ABSTRACT: Wing imaginal discs were dissected to generate body wall and wing/hinge fragments. Targets from three biological replicates of each were generated and the expression profiles were determined using Affymetrix Drosophila Genechip 1 arrays. Comparisons between the sample groups allow the identification of genes with localized expression patterns.

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Amanda Simcox 

PROVIDER: E-GEOD-93 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Discovery of genes with highly restricted expression patterns in the Drosophila wing disc using DNA oligonucleotide microarrays.

Butler Miranda J MJ   Jacobsen Thomas L TL   Cain Donna M DM   Jarman Michael G MG   Hubank Michael M   Whittle J Robert S JR   Phillips Roger R   Simcox Amanda A  

Development (Cambridge, England) 20030201 4

The Drosophila wing disc is divided along the proximal-distal axis into regions giving rise to the body wall (proximal), wing hinge (central) and wing blade (distal). We applied DNA microarray analysis to discover genes with potential roles in the development of these regions. We identified a set of 94 transcripts enriched (two fold or greater) in the body wall and 56 transcripts enriched in the wing/hinge region. Transcripts that are known to have highly restricted expression patterns, such as  ...[more]

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