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SiRNA knockdown in human HEK293 cells transfected with siRNA specific for StarPap or PIPKIalpha or control siRNA

ABSTRACT: A loss of StarPap would be predicted to result in a decrease in cellular levels of mRNAs which it polyadenylates. Moreover, if PIPKIalpha has a function relationship with StarPap, knockdown of PIPKIalpha should cause a decrease in a pool of target mRNAs which require both StarPap and PIPKIalpha for their maturation. To test this, we independently knocked down StarPap and PIPKIalpha, and performed microarray analysis of total polyadenylated mRNAs from each group. Experiment Overall Design: HEK293 cells were transfected with siRNA specific for StarPap or PIPKIalpha or control siRNA. N=3

ORGANISM(S): Homo sapiens

SUBMITTER: Christy Barlow 

PROVIDER: E-GEOD-9361 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

A PtdIns4,5P2-regulated nuclear poly(A) polymerase controls expression of select mRNAs.

Mellman David L DL   Gonzales Michael L ML   Song Chunhua C   Barlow Christy A CA   Wang Ping P   Kendziorski Christina C   Anderson Richard A RA  

Nature 20080201 7181

Phosphoinositides are a family of lipid signalling molecules that regulate many cellular functions in eukaryotes. Phosphatidylinositol-4,5-bisphosphate (PtdIns4,5P2), the central component in the phosphoinositide signalling circuitry, is generated primarily by type I phosphatidylinositol 4-phosphate 5-kinases (PIPKIalpha, PIPKIbeta and PIPKIgamma). In addition to functions in the cytosol, phosphoinositides are present in the nucleus, where they modulate several functions; however, the mechanism  ...[more]

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