Gene expression level during L-threonine production
Ontology highlight
ABSTRACT:
ORGANISM(S): Escherichia coli
SUBMITTER: Sang Yup Lee
PROVIDER: E-GEOD-9434 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Publications
Systems metabolic engineering of Escherichia coli for L-threonine production.
Lee Kwang Ho KH Park Jin Hwan JH Kim Tae Yong TY Kim Hyun Uk HU Lee Sang Yup SY
Molecular systems biology 20071204
Amino-acid producers have traditionally been developed by repeated random mutagenesis owing to the difficulty in rationally engineering the complex and highly regulated metabolic network. Here, we report the development of the genetically defined L-threonine overproducing Escherichia coli strain by systems metabolic engineering. Feedback inhibitions of aspartokinase I and III (encoded by thrA and lysC, respectively) and transcriptional attenuation regulations (located in thrL) were removed. Path ...[more]
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