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Transcription profiling of mouse gonocytes isolated from 2 dpp mouse testes by THY1+ MACS sorting and treated with retinoic acid

ABSTRACT: to investigate the RA regulated genes in 2 dpp thy1+ gonocytes Experiment Overall Design: gonocytes were isolated from 2 dpp mouse testes by THY1+ MACS sorting and cultured without serum, growth factors and feeder cells for 8hr, with either vehicle ethanol or RA (100 nM). Two control and two RA treated samples were included.

ORGANISM(S): Mus musculus

SUBMITTER: Lizhong Yang

PROVIDER: E-GEOD-9521 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Expression of stimulated by retinoic acid gene 8 (Stra8) and maturation of murine gonocytes and spermatogonia induced by retinoic acid in vitro.

Zhou Qing Q Li Ying Y Nie Rong R Friel Patrick P Mitchell Debra D Evanoff Ryan M RM Pouchnik Derek D Banasik Brent B McCarrey John R JR Small Christopher C Griswold Michael D MD

Biology of reproduction 20071121 3

Vitamin A deficiency in the mouse results in an arrest in the progression of undifferentiated spermatogonia to differentiating spermatogonia. The supplement of retinol to vitamin-A-deficient mice reinitiates spermatogenesis in a synchronous manner throughout the testes. It is unclear whether the effects of retinoids are the result of a direct action on germ cells or are indirectly mediated through Sertoli cells. The expression of Stimulated by retinoic acid gene 8 (Stra8), which is required for ...[more]

PMID: 18032419

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