Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Transcription profiling of rat embryonic kidney timepoints and a budded WD-MM recombination tissue

ABSTRACT: Here we compared the expression of an engineered kidney tissue, created by recombining an in vitro budded Wolffian duct with fresh E13 metanephric mesenchyme, with that of three in vivo rat embryonic kidney timepoints (E13, E18, and week 4) Experiment Overall Design: The global gene expression of recombined tissue (non-branched in vitro-formed UBs and MM) was analyzed and compared to the gene expression of early, late, and post developmental kidneys in order to determine whether normal developmental pathways were being followed. Genes with at least a 3-fold difference in expression between any of the 4 conditions (E13, E18, Wk4, recombined tissue) were analyzed and grouped into one of ten expression patterns. The expression of each group of genes was analyzed in the recombined tissue to compare the recombined tissue gene expression levels to the three in vivo time points.

ORGANISM(S): Rattus norvegicus

SUBMITTER: Sanjay Nigam 

PROVIDER: E-GEOD-9570 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Json Xml
altmetric image

Publications

Staged in vitro reconstitution and implantation of engineered rat kidney tissue.

Rosines E E   Sampogna R V RV   Johkura K K   Vaughn D A DA   Choi Y Y   Sakurai H H   Shah M M MM   Nigam S K SK  

Proceedings of the National Academy of Sciences of the United States of America 20071217 52

A major hurdle for current xenogenic-based and other approaches aimed at engineering kidney tissues is reproducing the complex three-dimensional structure of the kidney. Here, a stepwise, in vitro method of engineering rat kidney-like tissue capable of being implanted is described. Based on the fact that the stages of kidney development are separable into in vitro modules, an approach was devised that sequentially induces an epithelial tubule (the Wolffian duct) to undergo in vitro budding, foll  ...[more]

Similar Datasets

Transcriptomics Expression data from embryonic rat kidney timepoints and a budded WD-MM recombination tissue
2007-11-30 | GSE9570 | GEO
Transcriptomics Dissecting Stages of the Notch signaling pathway during Kidney Development by Oligonucleotide Array Sequence Analysis
2011-04-20 | GSE28496 | GEO
Transcriptomics Dissecting Stages of the Notch signaling pathway during Kidney Development by Oligonucleotide Array Sequence Analysis
2011-04-20 | E-GEOD-28496 | biostudies-arrayexpress
Transcriptomics Molecular Signatures and Cellular Diversity During Mouse Habenula Development
2022-02-16 | GSE188712 | GEO
Transcriptomics Transcription profiling of rat kidney progenitor tissue from embryo at E13
2009-12-19 | E-GEOD-18260 | biostudies-arrayexpress
Genomics Rattus norvegicus
| PRJNA103395 | ENA
Genomics Rattus norvegicus
| PRJNA117931 | ENA
Transcriptomics Expression data for kidney progenitor tissue from rat embryo at E13
2009-12-10 | GSE18260 | GEO
Genomics Mus musculus
| PRJNA219355 | ENA
Transcriptomics The Heterogeneous Nuclear Ribonucleoprotein U (HNRNPU) is the Safeguard of Neural Stem Cell Viability
2022-06-07 | GSE181527 | GEO