Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Comparison between saprotrophic and biotrophic-like mycelia of cacao WBD causal agent Moniliophthora perniciosa II

ABSTRACT: Moniliophthora perniciosa is the causal agent of cacao WitcheÂ´s broom disease. This disease has been causing extensive damages to Brazilian cacao plantation, especially in Southern Bahia. Using glass slides microarrays, we analyzed the expression profile of 3872 whole genome shotgun reads from M. perniciosa genome, comparing two stages of development (Biotrophic-like mycelia and saprotrophic mycelia). Keywords: Moniliophthora perniciosa, Witches Broom Disease, Pathogenesis, Cacao Pools from RNA of axenic cultures were amplified by RNA technique and labeled with Cyanine-3 CTP and Cyanine-5 CTP. Comparison was performed between biotrophic and necrotrophic mycelia, both grown in glycerol with cacao meristem extracts, using saprotrophic mycelia grown in glycerol as a control.

ORGANISM(S): Moniliophthora perniciosa

SUBMITTER: Johana Rincones

PROVIDER: E-GEOD-9627 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Differential gene expression between the biotrophic-like and saprotrophic mycelia of the witches' broom pathogen Moniliophthora perniciosa.

Rincones Johana J Scarpari Leandra M LM Carazzolle Marcelo F MF Mondego Jorge M C JM Formighieri Eduardo F EF Barau Joan G JG Costa Gustavo G L GG Carraro Dirce M DM Brentani Helena P HP Vilas-Boas Laurival A LA de Oliveira Bruno V BV Sabha Maricene M Dias Robson R Cascardo Júlio M JM Azevedo Ricardo A RA Meinhardt Lyndel W LW Pereira Gonçalo A G GA

Molecular plant-microbe interactions : MPMI 20080701 7

Moniliophthora perniciosa is a hemibiotrophic fungus that causes witches' broom disease (WBD) in cacao. Marked dimorphism characterizes this fungus, showing a monokaryotic or biotrophic phase that causes disease symptoms and a later dikaryotic or saprotrophic phase. A combined strategy of DNA microarray, expressed sequence tag, and real-time reverse-transcriptase polymerase chain reaction analyses was employed to analyze differences between these two fungal stages in vitro. In all, 1,131 putativ ...[more]

PMID: 18533830

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Project description:Background: Lactobacillus plantarum is found in a variety of fermented foods and as such, consumed for centuries. Some strains are natural inhabitants of the human gastro-intestinal tract and like other Lactobacillus species, L. plantarum has been extensively studied for its immunomodulatory properties and its putative health-promoting effects (probiotic). Being the first line of host defense intestinal epithelial cells (IEC) are key players in the recognition and initiation of responses to gut microorganisms. Results: Using high-density oligonucleotide microarrays we examined the gene expression profiles of differentiated Caco-2 cells exposed to various doses of L. plantarum. In addition, the effects were correlated to monolayer permeability studies and measurement of lactic acid production. A transcriptional dose-dependent IEC response to L. plantarum was found. Incubation of Caco-2 with a low bacterial dose induced a specific response, not due to cytotoxicity or production of lactic acid, including modulation of cell cycle and cell signaling functions. Exposure of Caco-2 cells to larger amounts of bacteria, accompanied by the production of lactic acid and glucose depletion, provoked increased permeability and supposed non-specific defense responses. Conclusions: These results suggest that IEC are able to sense and react to the presence of gut bacteria. This study provides the first description of global transcriptional response of human IEC to a commensal lactic acid bacterium, and it shows the importance of choosing physiological bacterial doses to prevent the observation of non-specific host reactions. Caco-2 cells were exposed for 10h to Lactobacillus. Fourteen samples are analyzed: 4 control Caco-2, 4 Caco-2 exposed to a low dose (10) of Lactobacillus, 4 Caco-2 exposed to a medium dose (100) of Lactobacillus, 2 Caco-2 exposed to a high dose (1000) of Lactobacillus. All 14 RNA samples are labeled with Cy5 and hybridized to a common reference (undifferentiated Caco-2, untreated) RNA labeled with Cy3

Dataset Information

Comparison between saprotrophic and biotrophic-like mycelia of cacao WBD causal agent Moniliophthora perniciosa II

Publications

Differential gene expression between the biotrophic-like and saprotrophic mycelia of the witches' broom pathogen Moniliophthora perniciosa.

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