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Terbutyl stress TR

ABSTRACT: Transcription rate (TR) analysis of W303-1a yeast strain growing in exponential phase in YPD subjected to terbutyl stress Keywords: time course Transcription rate analysis by means of GRO of three independent replicates the yeast strain growing in exponential phase. Each time point replicate has been hybridized on a different macroarray (F5-F10). A single DNA genomic hybridization from the same labeling reaction was done on the same macroarrays for normalization.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Jose Perez-Ortin

PROVIDER: E-GEOD-9663 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Comprehensive transcriptional analysis of the oxidative response in yeast.

Molina-Navarro María Micaela MM Castells-Roca Laia L Bellí Gemma G García-Martínez José J Marín-Navarro Julia J Moreno Joaquín J Pérez-Ortín José E JE Herrero Enrique E

The Journal of biological chemistry 20080417 26

The oxidative stress response in Saccharomyces cerevisiae has been analyzed by parallel determination of mRNA levels and transcription rates for the entire genome. A mathematical algorithm has been adapted for a dynamic situation such as the response to stress, to calculate theoretical mRNA decay rates from the experimental data. Yeast genes have been grouped into 25 clusters according to mRNA level and transcription rate kinetics, and average mRNA decay rates have been calculated for each clust ...[more]

PMID: 18424442

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