E-GEOD-9712 - Transcription profiling of human radioresistant tumor xenografts from athymic nude mice to detect genes differentially expressed in radioresistant tumors - OmicsDI

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcription profiling of human radioresistant tumor xenografts from athymic nude mice to detect genes differentially expressed in radioresistant tumors

ABSTRACT: Nu61, a radiation-resistant human tumor xenograft, was selected from a parental radiosensitive tumor SCC-61 by eight serial cycles of passage in athymic nude mice and in vivo irradiation. Obtained tumors were profiled using Affymetrix U133A arrays. Most abundant gene pattern associated with radioresistant phenotype was presented by IFN-inducible, Stat1-dependent pathway Experiment Overall Design: Obtained tumors were established as xenografts in nude mice. RNA were purified from each xenograft, normalized by concentration and three samples of RNA from each group were pooled for hybridization with each array. Three arrays were used for each group.

ORGANISM(S): Homo sapiens

SUBMITTER: Nikolai Khodarev

PROVIDER: E-GEOD-9712 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

STAT1 is overexpressed in tumors selected for radioresistance and confers protection from radiation in transduced sensitive cells.

Khodarev Nikolai N NN Beckett Michael M Labay Edwardine E Darga Thomas T Roizman Bernard B Weichselbaum Ralph R RR

Proceedings of the National Academy of Sciences of the United States of America 20040130 6

Nu61, a radiation-resistant human tumor xenograft, was selected from a parental radiosensitive tumor SCC-61 by eight serial cycles of passage in athymic nude mice and in vivo irradiation. Replicate DNA array experiments identified 52 genes differentially expressed in nu61 tumors compared with SCC-61 tumors. Of these, 19 genes were in the IFN-signaling pathway and moreover, 25 of the 52 genes were inducible by IFN in the nu61 cell line. Among the genes involved in IFN signaling, STAT1alpha and ST ...[more]

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