Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Transcription profiling of early development of neural crest stem cells and developing Schwann cells, from embryonic day 9.5 to postnatal day 0.


ABSTRACT: Time course of early development of peripheral nerve, from embryonic day 9.5 to postnatal day 0. Origin of samples: mouse embryos expressing green fluorescent protein (GFP) in neural crest stem cells and later glial cells under the control of the proteolipid protein gene (Plp) promoter. At E9.5 the trunk region was dissected out. At E12, E14, E16, E18 and P0 the sciatic nerve was taken. GFP-expressing cells were isolated by FACS sorting. Three or 4 separate batches of embryos analyzed at each stage.

ORGANISM(S): Mus musculus

SUBMITTER: Ned Mantei 

PROVIDER: E-GEOD-972 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

altmetric image

Publications

Efficient isolation and gene expression profiling of small numbers of neural crest stem cells and developing Schwann cells.

Buchstaller Johanna J   Sommer Lukas L   Bodmer Matthias M   Hoffmann Reinhard R   Suter Ueli U   Mantei Ned N  

The Journal of neuroscience : the official journal of the Society for Neuroscience 20040301 10


Schwann cells develop from multipotent neural crest stem cells and are important for neuronal survival, maintenance of axonal integrity, and myelination. We used transgenic mice expressing green fluorescent protein in a tissue-specific manner to isolate viable, pure populations of neural crest stem cells and developing Schwann cells, which are not readily accessible by microdissection. Starting with the minute amounts of RNA obtained, a two-round amplification procedure was used to achieve repro  ...[more]

Similar Datasets

2004-02-21 | GSE972 | GEO
2022-02-16 | GSE188712 | GEO
2021-04-15 | GSE172110 | GEO
2020-12-03 | GSE162502 | GEO
2021-02-16 | GSE143806 | GEO
2011-04-20 | GSE28496 | GEO
2011-04-20 | E-GEOD-28496 | biostudies-arrayexpress
2014-08-01 | E-GEOD-57505 | biostudies-arrayexpress
2022-03-16 | PXD028131 | JPOST Repository
2008-06-01 | E-MEXP-1478 | biostudies-arrayexpress