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A Time Course Analysis of the Effects of Marinobufagenin in Human Dermal Fibroblasts

ABSTRACT: Gene expression profiling of the effets of marinobufagenin in a time-course study (0, 0.25, 0.5, 0.75, 1, 2, 3, 6, 9, 12, 15, and 24 hrs) to examine the temporal changes in gene expression in a human dermal fibroblast cell line. Keywords: Time course Cells were treated with 10 nM of marinobufagenin (MBG) for the following times: 0, 0.25, 0.5, 0.75, 1, 2, 3, 6, 9, 12, 15, and 24 hrs, and then cells were harvested for RNA isolation for gene expression analysis.

ORGANISM(S): Homo sapiens

SUBMITTER: Khew-Voon Chin

PROVIDER: E-GEOD-9806 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Effects of cardiotonic steroids on dermal collagen synthesis and wound healing.

El-Okdi Nasser N Smaili Sleiman S Raju Vanamala V Shidyak Amjad A Gupta Shalini S Fedorova Larisa L Elkareh Jihad J Periyasamy Sankaridrug S Shapiro Anna P AP Kahaleh M Bashar MB Malhotra Deepak D Xie Zijian Z Chin Khew Voon KV Shapiro Joseph I JI

Journal of applied physiology (Bethesda, Md. : 1985) 20080515 1

We previously reported that cardiotonic steroids stimulate collagen synthesis by cardiac fibroblasts in a process that involves signaling through the Na-K-ATPase pathway (Elkareh et al. Hypertension 49: 215-224, 2007). In this study, we examined the effect of cardiotonic steroids on dermal fibroblasts collagen synthesis and on wound healing. Increased collagen expression by human dermal fibroblasts was noted in response to the cardiotonic steroid marinobufagenin in a dose- and time-dependent fas ...[more]

PMID: 18483172

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