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Transcription profiling of chicken circulating red blood cells and non red blood cells in the chicken embryo (E4 and E6)

ABSTRACT: Blood was extracted from embryonic hearts at E4 and E6 and non-red blood was separated by density gradient centrifugation Experiment Overall Design: There are a total of 9 samples which have one biological replicate each, except negative control E6 embryonic heart (used to screen heart contamination in our blood samples - which were drawn from the heart) which has only one sample.

ORGANISM(S): Gallus gallus

SUBMITTER: Brendan McIntyre 

PROVIDER: E-GEOD-9884 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Expression profiling of circulating non-red blood cells in embryonic blood.

McIntyre Brendan A S BA   Alev Cantas C   Tarui Hiroshi H   Jakt Lars M LM   Sheng Guojun G  

BMC developmental biology 20080227

<h4>Background</h4>In addition to erythrocytes, embryonic blood contains other differentiated cell lineages and potential progenitor or stem cells homed to changing niches as the embryo develops. Using chicken as a model system, we have isolated an enriched pool of circulating non red blood cells (nRBCs) from E4 and E6 embryos; a transition period when definitive hematopoietic lineages are being specified in the peri-aortic region.<h4>Results</h4>Transcriptome analysis of both nRBC and RBC enric  ...[more]

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