Unknown,Transcriptomics,Genomics,Proteomics

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Rlm1 mutant 3 hour exposure to zymolyase

ABSTRACT: We did transcription profiling on the effect of rlm1 deletion, gene involved in cell wall stress response Keywords: cell wall stress response S. cerevisiae (rlm1 mutant) was grown on YEPD. For Zymolyase experiments, yeast cells were grown overnight at 24 Â°C to an optical density 0.8 - 1 (A600). The culture was refreshed to 0.2 O.D and grown at 24 Â°C for 2h 30min. Next, culture was divided into two parts. One continues growing under same conditions (non-treated culture) while the other was supplemented with 5units/ml Zymolyase 100T. Cells were collected at 3 hours of growth, frozen at -80 Â°C and processed for RNA extraction. The total RNA from two different cultures was analyzed, and, in addition, for each sample two different hybridizations were performed, including fluorochrome swapping in order to minimize transcriptional changes due to technical variability. This therefore corresponded to four DNA microarrays analyzed for each condition.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Javier Arroyo

PROVIDER: E-GEOD-9933 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

The high osmotic response and cell wall integrity pathways cooperate to regulate transcriptional responses to zymolyase-induced cell wall stress in Saccharomyces cerevisiae.

García Raúl R Rodríguez-Peña Jose M JM Bermejo Clara C Nombela César C Arroyo Javier J

The Journal of biological chemistry 20090220 16

The adaptation of Saccharomyces cerevisiae to situations in which cell wall integrity is seriously compromised mainly involves the cell wall integrity (CWI) pathway. However, in a recent work ( Bermejo, C., Rodriguez, E., García, R., Rodríguez-Peña, J. M., Rodríguez de la Concepción, M. L., Rivas, C., Arias, P., Nombela, C., Posas, F., and Arroyo, J. (2008) Mol. Biol. Cell 19, 1113-1124 ) we have demonstrated the co-participation of the high osmotic response (HOG) pathway to ensure yeast surviva ...[more]

PMID: 19234305

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Dataset Information

Rlm1 mutant 3 hour exposure to zymolyase

Publications

The high osmotic response and cell wall integrity pathways cooperate to regulate transcriptional responses to zymolyase-induced cell wall stress in Saccharomyces cerevisiae.

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