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Transcription profiling of planktonic pseudomonas aeruginosa exposed to tobramycin treatment

ABSTRACT: As a comparison to tobramycin-treated P. aeruginosa biofilms, we investigated the response of planktonic P. aeruginosa to tobramycin by microarray. Experiment Overall Design: We included 2 control (untreated) cultures and 2 tobramycin-treated cultures. We used mid-exponential phase cultures of P. aeruginosa PA14. Replicate cultures were incubated in the presence or absence of 5 Î¼g/mL tobramycin for 30 minutes at 37Â°C.

ORGANISM(S): Pseudomonas aeruginosa

SUBMITTER: George O'Toole

PROVIDER: E-GEOD-9991 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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In vitro analysis of tobramycin-treated Pseudomonas aeruginosa biofilms on cystic fibrosis-derived airway epithelial cells.

Anderson Gregory G GG Moreau-Marquis Sophie S Stanton Bruce A BA O'Toole George A GA

Infection and immunity 20080122 4

P. aeruginosa forms biofilms in the lungs of individuals with cystic fibrosis (CF); however, there have been no effective model systems for studying biofilm formation in the CF lung. We have developed a tissue culture system for growth of P. aeruginosa biofilms on CF-derived human airway cells that promotes the formation of highly antibiotic-resistant microcolonies, which produce an extracellular polysaccharide matrix and require the known abiotic biofilm formation genes flgK and pilB. Treatment ...[more]

PMID: 18212077

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Transcription profiling of Pseudomonas aeruginosa treated with tobramycin

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