Dataset Information
Transcription profiling by array of E. coli BL21(DE3)::TN7T7 -SOD to analyze the cellular response of this plasmid-free expression system to high level expression of recombinant human super-oxide-dismutase
Ontology highlight
ABSTRACT:
ORGANISM(S): Escherichia coli
SUBMITTER: Peter Krempl
PROVIDER: E-MARS-24 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Publications
Comparative transcription profiling and in-depth characterization of plasmid-based and plasmid-free Escherichia coli expression systems under production conditions.
Applied and environmental microbiology 20130412 12
Plasmid-based Escherichia coli BL21(DE3) expression systems are extensively used for the production of recombinant proteins. However, the combination of a high gene dosage with strong promoters exerts extremely stressful conditions on producing cells, resulting in a multitude of protective reactions and malfunctions in the host cell with a strong impact on yield and quality of the product. Here, we provide in-depth characterization of plasmid-based perturbations in recombinant protein production ...[more]
