Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcription profiling of Medicago truncatula cell suspension treated with yeast elicitor or methyl jasminate for 2 or 24 hrs to compare early and late responses

ABSTRACT: Cell suspensions were treated with yeast elicitor (pathogen) or methyl jasmonate (wounding signal) and harvested at 2 and 24 hour time points in order to compare the transcriptomics between them in early and late response.

ORGANISM(S): Medicago truncatula

SUBMITTER: Yuhong Tang

PROVIDER: E-MEXP-1092 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Genomic and coexpression analyses predict multiple genes involved in triterpene saponin biosynthesis in Medicago truncatula.

Naoumkina Marina A MA Modolo Luzia V LV Huhman David V DV Urbanczyk-Wochniak Ewa E Tang Yuhong Y Sumner Lloyd W LW Dixon Richard A RA

The Plant cell 20100326 3

Saponins, an important group of bioactive plant natural products, are glycosides of triterpenoid or steroidal aglycones (sapogenins). Saponins possess many biological activities, including conferring potential health benefits for humans. However, most of the steps specific for the biosynthesis of triterpene saponins remain uncharacterized at the molecular level. Here, we use comprehensive gene expression clustering analysis to identify candidate genes involved in the elaboration, hydroxylation, ...[more]

PMID: 20348429

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Project description:Anthocyanins are flavonoid compounds responsible for red/purple colours in the leaves, fruit and flowers of many plant species. They are produced through a multistep pathway which is controlled by MYB transcription factors. VvMYBA1 and VvMYBA2 activate anthocyanin biosynthesis in grapevine (Vitis vinifera) and are non-functional in white grapevine cultivars. In this study, transgenic grapevines with altered VvMYBA gene expression were developed, and transcript analysis was carried out on berries using a microarray technique. The results showed that VvMYBA is a positive regulator of the later stages of anthocyanin synthesis, modification and transport in Shiraz. One up-regulated gene ANTHOCYANIN 3- O-GLUCOSIDE-6â-O-ACYLTRANSFERASE (Vv3AT) encodes a BAHD acyltransferase protein, belonging to a clade separate from most anthocyanin acyltransferases. Functional studies (in planta and in vitro) show that Vv3AT has a broad anthocyanin substrate specificity and can also utilise both aliphatic and aromatic acyl donors, a novel activity for this enzyme family found in nature. In V. vinifera cv. Pinot Noir, a red-berried grapevine mutant lacking acylated anthocyanins, Vv3AT contains a nonsense mutation encoding a truncated protein that lacks two motifs required for BAHD protein activity. Promoter activation assays confirm that Vv3AT transcription is activated by VvMYBA1, which adds to the current understanding of the regulation of the BAHD gene family. The flexibility of Vv3AT to use both classes of acyl donors will be useful in the engineering of anthocyanins in planta or in vitro. Transgenic Chardonnay/Shiraz and non-transformed WT controls were all grown in the same glasshouse in ambient light with a night break. Day and night temperatures were approximately 27oC and 22oC respectively. For microarray experiments, whole berries were sampled from independent transgenic lines: three from transgenic Chardonnay and four from transgenic Shiraz, resulting in three and four biological replicates respectively. Bunches were harvested close to ripeness based on average total soluble solids (TSS, measured as oBrix). This was aimed to be between 20 â 24 oBrix and was determined from TTS of a subsamples from each bunch (Table S53). A sample consisted of all remaining berries from a single bunch except when there were <100 berries in which case more than one bunch was used in the one replicate. Whole berries were immediately frozen in liquid nitrogen. For skin samples, the skins were first removed from fresh berries then frozen in liquid nitrogen. All samples were stored at -80oC.

Dataset Information

Transcription profiling of Medicago truncatula cell suspension treated with yeast elicitor or methyl jasminate for 2 or 24 hrs to compare early and late responses

Publications

Genomic and coexpression analyses predict multiple genes involved in triterpene saponin biosynthesis in Medicago truncatula.

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