Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of leaves from wild type, fou2 mutant and tpc1-2 mutant Arabidopsis plants


ABSTRACT: Compare gene expression in the resting leaves of Arabidopsis thaliana WT with the mutants fou2 and tpc1-2. To analyse the contribution of the fou2 and tpc1-2 mutations in Arabidopsis thaliana to gene expression, transcript levels in the resting leaves of Arabidopsis were measured using 4 weeks-old plants grown in control conditions (long day).

INSTRUMENT(S): ScanArray 4000 [PerkinElmer]

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Edward Farmer 

PROVIDER: E-MEXP-1095 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

The fou2 gain-of-function allele and the wild-type allele of Two Pore Channel 1 contribute to different extents or by different mechanisms to defense gene expression in Arabidopsis.

Bonaventure Gustavo G   Gfeller Aurélie A   Rodríguez Víctor M VM   Armand Florence F   Farmer Edward E EE  

Plant & cell physiology 20071102 12


The fatty acid oxygenation up-regulated 2 (fou2) mutant in Arabidopsis thaliana creates a gain-of-function allele in a non-selective cation channel encoded by the Two Pore Channel 1 (TPC1) gene. This mutant genetically implicates cation fluxes in the control of the positive feedback loop whereby jasmonic acid (JA) stimulates its own synthesis. In this study we observed extensive transcriptome reprogramming in healthy fou2 leaves closely resembling that induced by treatment with methyl jasmonate,  ...[more]

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