Transcription profiling of S. cerevisiae (budding yeast) mutants reveals Isc1p plays a key role in hydrogen peroxide resistance and chronological lifespan through modulation of iron levels and programmed cell death

ABSTRACT: Changes in the transcriptome of Isc1p deficient mutants. The inositolphosphosphingolipid phospholipase C (Isc1p) of Saccharomyces cerevisiae belongs to the family of neutral sphingomyelinases that generates the bioactive sphingolipid ceramide. In this work the role of Isc1p in oxidative stress resistance and chronological lifespan was investigated. Loss of Isc1p resulted in a higher sensitivity to hydrogen peroxide that was associated with an increase in oxidative stress markers, namely intracellular oxidation, protein carbonylation and lipid peroxidation. Microarray analysis showed that Isc1p deficiency upregulated the iron regulon leading to increased levels of iron, which is known to catalyze the production of the highly reactive hydroxyl radicals via the Fenton reaction. In agreement,

INSTRUMENT(S): Scanning hardware

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Vitor Costa 

PROVIDER: E-MEXP-1136 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress