Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

34 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcription profiling of in vivo range finding studies in the rat with selected individual food additives

ABSTRACT: FSAT01021/OBJ01/28D:
TITLE: Development of methods for the assessment of the health effects from mixtures of food additives. SAP/S3 project code: 010.45073
TNO Voeding-TNO BIBRA project for UK Food Standard Agency
Objective No. 01: In vivo range finding studies in the rat with selected individual food additives.

Rat study using 4 compounds, oral administration in diet (concentration given in ppm), for 28 days:
Butylated hydroxytoluene (BHT): 254 ppm; 762 ppm; 1523 ppm; 3046 ppm; 10154 ppm
Curcumin (CC): 254 ppm; 762 ppm; 2031 ppm; 4062 ppm; 10154 ppm
Propyl Gallate (PG): 203 ppm; 508 ppm; 1523 ppm; 3046 ppm; 6092 ppm
Thiabendazole (TB): 102 ppm; 254 ppm; 762 ppm; 2031 ppm; 5077 ppm

Within Objective No. 01: clinical chemistry, pathology, drug metabolizing enzymes are assessed as well.

Within Objective No. 01: A smaller study in which rats were exposed for 3 days to two doses of BHT, CC, PG, TB was performed as well. Depending on the outcome of arrays performed within FSAT01021/OBJ01/28D, transcriptomics will be performed on these samples: the Experiment name then will be FSAT01021/OBJ01/3D

for details: Rob Stierum , AVTO, Monday December 17th 2001

ORGANISM(S): Rattus norvegicus

SUBMITTER: Marinus Dansen

PROVIDER: E-MEXP-116 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Subventricular zone/white matter microglia reconstitute the empty adult microglial niche in a dynamic wave

Project description:28d Regular Repop Study: Two-month-old male C57BL/6 (Jax 000664) were maintained on control diet for 35 days (control) or treated for 7 days with PLX3397 (600 ppm) diet, then to stimulate repopulation PLX3397 placed with control diet for 28 days (28d Recovery). Mice were sacrificed and myeloid cells were extracted from whole hemispheres. RNA was extracted from single-cell suspensions generated from fluorescence-activated cell sorting (FACS) gating for CD11b+CD45int cells. SVZ Study: Two-month-old male C57BL/6 (Jax 000664) were maintained on control diet for 19 days (control), treated for 14 days with PLX3397 (600 ppm) diet (microglial elimination), or treated for 14 days with PLX3397 (600 ppm) diet and then placed on control diet for 5 days (5d recovery). Mice were sacrificed and the subventricular zone was micro-dissected for RNA isolation. 28d Alt Repop +/- LPS Study: Two-month-old male C57BL/6 (Jax 000664) were maintained on control diet for 35 days (control) or treated for 14 days with PLX3397 (600 ppm) diet and then placed on control diet for 28 days (28d Recovery). At 6 or 24 hours (hr) prior to sacrifice, lipopolysaccharide (LPS; 0.33 mg/kg body weight) was administered via intraperitoneal injection. Some mice did not receive an LPS injection and serve as controls (0 hr). Mice were sacrificed and myeloid cells were extracted from whole hemispheres. RNA was extracted from single-cell suspensions generated from fluorescence-activated cell sorting (FACS) gating for CD11b+CD45int cells.

2021-02-04 | GSE166092 | GEO

Cellular reactions to long-term volatile organic compound (VOC) exposures.

Project description:A549 cells were grown at air liquid interphase (ALI) and exposed to airborne formaldehyde for three days. An exposure platform was developed for this purpose, which provided the volatile analyte in a humidified atmosphere. The platform was composed of a reference and an exposure chamber. Treatments/ Sample code: (A) 0 ppm (not = no treatment) in the exposure chamber and control cells in the reference chamber (co); (B) 0.1 ppm (01 FA) in the exposure chamber and control cells in the reference chamber (co); and (C) 0.5 ppm (05FA) in the exposure chamber and control cells in the reference chamber (co)

2016-12-05 | GSE76790 | GEO

Effect of treatment for three months with chemical carcinogen and non-carcinogens on liver miRNAome

Project description:Here, we examined the liver microRNA profile of male Fischer rats exposed through their diet to genotoxic (2-acetylaminofluorene) and epigenetic (phenobarbital, diethylhexylphthalate, methapyrilene HCL, monuron, and chlorendic acid) chemical hepatocarcinogens, as well as to non-hepatocarcinogenic treatments (benzophenone, and diethylthiourea) for three months. The aim of the study was to investigate how liver miRNA profiles relate to mode of action and carcinogenic potential of chemicals. In total miRNA profiles from 38 liver samples were examined. These included 3-5 samples for animal fed control diets or diet supplemented with designated chemicals. For each chemical a carcinogenic or a maximum tolerated dose was chosen based on published literature. The treatments were Diethylthiourea (DETU) 2500 ppm; Benzophenone (BP) 1250 ppm ; Monuron (MON) 1500 ppm ; Diethylhexylphthalate (DEHP) 12000 ppm ; Phenobarbital (PB) 1000 ppm; Chlorendic acid (CA) 1250 ppm; Methapyrilene HCl (MP) 250 ppm; 2-Acetyl aminofluorene (2-AAF) 40 ppm.

2013-07-03 | E-GEOD-48492 | biostudies-arrayexpress

miR-762 overexpression effect on tongue cancer cells

Project description:Analysis of SCC-15 tongue cancer cells overexpressing miR-762. miR-762 is frequently upregulated in primary tongue cancer. Results provide insight into the role of miR-762 in the pathogenesis of tongue cancer. We used micorarrays to detailed the genes regulated by miR-762 in SCC-15 cells.

2018-11-09 | GSE122328 | GEO

Investigating RDX neurotoxicity in rat brain using gene expression analysis and transcriptional network modeling

Project description:ABSTRACT: The central nervous system is remarkably plastic in its ability to recover from trauma. We examined recovery from hexahydro-1,3,5-trinitrotriazine (RDX) induced seizures in rat through changes in transcriptional networks. Transcriptional networks from time series experiments provide a good basis for organizing and studying the dynamic behavior of biological processes. The goal of this work was to identify networks affected by chemical exposure and track changes in these networks as animals recover. We examined brain microarray data from rats exposed to 0, 1.2, 12, 24, and 47 mg RDX/kg body weight at different time points after exposure (24hr, 48hr, 7d, 14d, 28d and 90d). RESULTS A credible transcriptional network was constructed from the gene expression microarray data, which predicts the role of some key genes such as heat shock proteins, neuropeptide Y, thyrotropin-releasing hormones, growth factors, and ion channels in neurotransmission and neuroprotective mechanisms. Examination of the dynamic changes in expression within this network over time provided insight into CNS protective mechanisms from traumas. Single RDX Exposure with Various Time Points, Brain Tissue Investigation: Sprague-Dawley female rats were exposed to a single oral gavage of one of four concentrations of RDX or vehicle control with sampling periods of 24h, 48h, 7d, 14d, 28d, or 90d. Brain tissue was investigated for differential expression in response to RDX exposure and provide insight into CNS protective mechanisms associated with RDX exposure.

2011-05-31 | E-GEOD-27042 | biostudies-arrayexpress

Bacillus velezensis strain:BR-01

Project description:Bacillus velezensis BR-01 genome sequencing

| PRJNA792055 | ENA

Transcription profiling of Rattus norvegicus hippocampus cells under prenatal stress

Project description:The study determined whether there were gender differences in the expression of hippocampal genes in adult rats in association with dissimilarity in their behavior, and how these were affected by prenatal stress. Pregnant Wistar rats were subjected to varied stress once daily on days 14-20 of gestation.

2010-01-01 | E-MEXP-2470 | biostudies-arrayexpress

Transcription profiling of rat sarcopenic skeletal muscle after treatment with extract of Ginko biloba Egb 761 shows that a juvenile gene expression profile is reactivated

Project description:The sarcopenia-associated decline of the muscular performances in older people is improved by exercise and caloric restriction but is still lacking a more suitable pharmacological treatment. We investigated the effects of EGb 761, a Gingko biloba extract used to treat chronic age-dependent neurological disorders, on the soleus muscle of aged rats. DNA microarray analysis shows that the EGb 761 treatment is associated with the transcriptional reprogramming of genes related to regeneration and energy production functions. EGb 761 up-regulated genes implicated in myogenesis though the TGF? signaling pathway. Also, EGb 761, by modulating genes implicated in fatty acids and glucose oxidation, reversed the agerelated shift from lipids to glucose and improved energy production in mitochondria. These modifications restore a more juvenile gene expression pattern that is concomitant with the improvement of the muscular performances, as assessed by electrophysiological and biochemical parameters. Thus, EGb 761 may represent a novel treatment for sarcopenia

2009-06-30 | E-MEXP-1937 | biostudies-arrayexpress

Dose and temporal effects of Phenobarbital on the liver miRNAome of male Fischer rats

Project description:Male Fischer rats were fed a control diet or a diet supplemented with 50, 500 or 1000 ppm Phenobarbital. The 500 and 1000 ppm PB doses induced a transient hyperplasia and a sustained hepatomegaly in treated animals. The animals were sacrificed and liver miRNAome was profiled in three animals per group after 1, 3, 7, and 14 days of treatment. The aim was to investigate the time and dose dependent effects of phenobarbital treatment on the liver miRNAome Three animals were profiled from control or each treatment group after 1, 3, 7, and 14 days

2013-07-03 | E-GEOD-48489 | biostudies-arrayexpress

MicroRNA profiling of human cholangiocyte H69 cells after culturing with lipopolysaccharide

Project description:H69 cells were cultured in H69 medium with 1 ng/ml lipopolysaccharide(LPS, for smaples 04, 05 and 06) or without LPS(for samples 01, 02 and 03) for 8 hours and then collected for array analysis.

2010-02-11 | E-MEXP-2052 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data