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Unknown,Transcriptomics,Genomics,Proteomics

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Chromatin immunoprecipitation of GCN5 target sites in Arabidopsis gcn5 mutant and wild type seedlings

ABSTRACT: GCN5 plays an essential role in chromatin modification and transcriptional regulation, its mode of action is not understood. It is therefore crucial to determine what are the sites targeted by GCN5 in the Arabidopsis genome to decipher the regulatory mechanisms in which it is involved. Array technologies have been used in recent years to determine, at the scale of whole genomes, the sites bound in vivo by chromatin-associated proteins (Hanlon and Lieb 2004) or targeted by chromatin-modifying enzymes (Millar and Grunstein 2006; Zhang et al. 2006). In this study we took advantage of the chromatin immunoprecipitation (ChIP) technique coupled with hybridization to a novel Arabidopsis promoter array to show that GCN5 is recruited to a large number of transcriptionally active promoters.

INSTRUMENT(S): Axon GenePix 4000A scanning hardware

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: ludivine taconnat

PROVIDER: E-MEXP-1233 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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