Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of zebrafish ovary, testis, brain, kidney and whole body to identify gonad-expressed genes and their expression in various organs


ABSTRACT: Our knowledge about the molecular regulation of zebrafish gonad development and function is very limited. This study aims to identify gonad-expressed genes in zebrafish and to analyze their expression in various organs. Using our custom ˜Gonad Uniclone Microarray v1, we performed a total of 32 hybridizations, using eight target organs with four biological replicates each. The target organs consisted of adult ovary and testis; the brain, kidney and rest-of-body (ie. whole body except the three organs listed earlier) from both male and female zebrafish, were used as controls. For simplicity, rest of body will be regarded as an organ. All targets were hybridized against a pooled common reference consisting of equal amounts of targets from all dissected organs from a single adult male and a single adult female.

INSTRUMENT(S): ScanArray Express HT [PerkinElmer]

ORGANISM(S): Danio rerio

SUBMITTER: Rajini Sreenivasan 

PROVIDER: E-MEXP-1272 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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