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Transcription profiling of four yeast strains grown in SC medium and shifted for 2.5h to 37C: YRA1 wild type, YRA1 mlp2916; GFP-yra1-8, and GFP-yra1-8 mlp2916

ABSTRACT: Comparison of mRNA levels in four yeast strains grown in SC medium and shifted for 2.5h to 37C: YRA1 wild-type, YRA1 mlp2Δ GFP-yra1-8, and GFP-yra1-8 mlp2Δ

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Francoise Stutz

PROVIDER: E-MEXP-130 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Perinuclear Mlp proteins downregulate gene expression in response to a defect in mRNA export.

Vinciguerra Patrizia P Iglesias Nahid N Camblong Jurgi J Zenklusen Daniel D Stutz Françoise F

The EMBO journal 20050203 4

The mRNA export adaptor Yra1p/REF contributes to nascent mRNP assembly and recruitment of the export receptor Mex67p. yra1 mutants exhibit mRNA export defects and a decrease in LacZ reporter and certain endogenous transcripts. The loss of Mlp1p/Mlp2p, two TPR-like proteins attached to nuclear pores, rescues LacZ mRNA levels and increases their appearance in the cytoplasm, without restoring bulk poly(A)+ RNA export. Chromatin immunoprecipitation, FISH and pulse-chase experiments indicate that Mlp ...[more]

PMID: 15692572

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