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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of male flounder isolated from different rivers

ABSTRACT: Comparison of environmentally sampled flounder from the Tyne and Alde estuaries, related experiment E-MEXP-15

ORGANISM(S): Platichthys flesus

SUBMITTER: Steven Minchin

PROVIDER: E-MEXP-14 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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A DNA expression array to detect toxic stress response in European flounder (Platichthys flesus).

Williams T D TD Gensberg K K Minchin S D SD Chipman J K JK

Aquatic toxicology (Amsterdam, Netherlands) 20031001 2

As a first stage in developing a DNA array-based approach to investigating the effects of pollutants on an environmentally relevant European fish species, we have constructed a 160-gene custom microarray for European flounder. Degenerate primers were used to amplify 110 different fragments of stress-related and other genes from European flounder cDNA and genomic DNA. Additionally, 22 fragments were obtained by suppressive subtractive hybridisation (SSH). These fragments were cloned and sequenced ...[more]

PMID: 12946615

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