Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcription profiling by array of S. cerevisiae wild type, SNF1, SNF4, and double knock outs to investigate SNF1/4 dependent gene expression

ABSTRACT: The protrotophic laboratory strain CEN.PK113-7D (MAT a) and three knock-out strains snf1, snf4 and snf1snf4 were grown in laboratory fermentors with a working volume of 1 litre at dilution rate (D) of 0.10 per hour (in triplicate for each strain). At steady state, samples from each of the 12 continuous cultures were taken and cooled below 2 degree C within ten seconds by mixing 40% sample and 60% crushed ice.

INSTRUMENT(S): GeneChip Scanner 3000 [Affymetrix]

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Renata Usaite

PROVIDER: E-MEXP-1407 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Reconstruction of the yeast Snf1 kinase regulatory network reveals its role as a global energy regulator.

Usaite Renata R Jewett Michael C MC Oliveira Ana Paula AP Yates John R JR Olsson Lisbeth L Nielsen Jens J

Molecular systems biology 20091103

Highly conserved among eukaryotic cells, the AMP-activated kinase (AMPK) is a central regulator of carbon metabolism. To map the complete network of interactions around AMPK in yeast (Snf1) and to evaluate the role of its regulatory subunit Snf4, we measured global mRNA, protein and metabolite levels in wild type, Deltasnf1, Deltasnf4, and Deltasnf1Deltasnf4 knockout strains. Using four newly developed computational tools, including novel DOGMA sub-network analysis, we showed the benefits of thr ...[more]

PMID: 19888214

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Dataset Information

Transcription profiling by array of S. cerevisiae wild type, SNF1, SNF4, and double knock outs to investigate SNF1/4 dependent gene expression

Publications

Reconstruction of the yeast Snf1 kinase regulatory network reveals its role as a global energy regulator.

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