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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of short-term cultured Myc transgenic vs. Myc transgenic Suv39h1 deficient mouse lymphoma cells with and without exposure to 100 pM of human TGF-b1

ABSTRACT: Genome-wide expression analysis was performed on RNA from short-term cultured Eµ-myc transgenic vs. Eµ-myc transgenic; Suv39h1-/- lymphoma cells with and without exposure to 100 pM of human TGF-b1 for 24 hours, with wildtype B-cells as control

INSTRUMENT(S): ScanArray Express HT [PerkinElmer]

ORGANISM(S): Mus musculus

SUBMITTER: Birgit Samans

PROVIDER: E-MEXP-1424 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcription profiling of whole lymph node RNA from mice with different Suv39h1 genotypes

Project description:Genome-wide expression analysis was performed on RNA from whole neoplastic lymph nodes derived from individual Eµ-myc transgenic and Eµ-myc transgenic; Suv39h1-/- mice and from normal wildtype spleens as control.

2008-02-20 | E-MEXP-1423 | biostudies-arrayexpress

Transcription profiling of mouseB-cell lymphomas with defined genetic alterations in p53 or p73

Project description:Induction of apoptosis by the tumor suppressor p53 is known to protect from Myc-driven lymphomagenesis. The p53 family member p73 is also a pro-apoptotic protein, which is activated in response to oncogenes like Myc. We therefore investigated whether p73 provides a similar protection from Myc-driven lymphomas as p53. To generate B-cell lymphomas with defined genetic alterations in p53 or p73, we crossed the Eµ-Myc transgenic to mice heterozygous for germ-line deletions in p53 (p53+/) or p73 (p73+/-). Lymphomas which have spontaneously developed in Eµ-Myc transgenic animals with the genotypes p53+/+, p53+/-, p73+/+, p73+/- or p73-/- were isolated when the animals were moribund and further processed for gene expression profiling with 22.5K cDNA microarrays.

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Transcription profiling of human Soas-2 cells infected with adenoviruses expressing p53 proteins with charge-neutralizing or charge-inverting mutations in the H1 helix to investigate the role of the dimerization interface in p53 function

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2010-04-01 | E-MEXP-1209 | biostudies-arrayexpress

Transcription profiling time series of mouse melanocytes transfected with an inducible version of the oncogenic receptor Xmrk, starved for 72 h and afterwards stimulated with EGF

Project description:Signaling pathways specifically induced by the oncogenic receptor Xmrk can be elucidated by comparing gene expression in cells with the activated receptor to gene expression in cells with the non-activated receptor. Mouse melanocytes transfected with an inducible version of the oncogenic receptor Xmrk, were starved for 72 h and afterwards stimulated with EGF. We compared cells stimulated for 15 minutes, 1 h, 2 h, 4 h, 8 h or 24 h, respectively, to unstimulated cells.

2008-08-31 | E-MEXP-1311 | biostudies-arrayexpress

RNAi knock down of AURKA in human neuroblastoma IMR-32 cells

Project description:IMR-32 cells were infected either with control-shRNA or one of two different AURKA-shRNA vectors to identify genes regulated after AURKA knockdown.

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siRNAi knockdown of ZBTB4 in human SH-EP cells reveals Zbtb4 represses transcription of P21CIP1 and controls the cellular response to p53 activation

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