Transcription profiling of mechanically stretched mouse NIH 3T3 cells to gain insights into the molecular and genetic basis of effect of mechanical stress

ABSTRACT: Type of experiment:
Our experiments examine gene expression profiles from untreated and mechanical stretching NIH 3T3 cells to gain new insights about the molecular and genetic basis of effect of mechanical stress.

Experimental factors:
We compared differences in gene expression profiles between 0.25% mechanical strain and control factor for NIH 3T3 cells.

The number of hybridizations performed in the experiment:
A total of 4 hybridizations were performed on Agilent One-color microarray platform.

Number of replicates (Biological or Technical)
3 biological replicates for treated condition

Hybridization design:
All treated NIH 3T3 cells sample RNAs were hybridized against a pooling control RNA sample (see above). Cells were cultured in a humidifier incubator at 37 0C and 5% CO2. After cells were confluent, these cells were subjected to cyclic sinusoidal strain at 0.25 Hz for 60 minutes at a maximum membrane indentation of 1 mm., equal to 0.25% strain. Strain experiments were carried out in a humidified incubator with 5% CO2 at 37°C. Control cells were treated identical, however, not exposed to mechanical stress.

INSTRUMENT(S): G2565BA DNA microarray scanner [Agilent]

ORGANISM(S): Mus musculus

SUBMITTER: taylan demirci 

PROVIDER: E-MEXP-1462 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress